Antibody Detection and Identification

Case Study One- Immune Alloantibody

A 42 year old male received 6 units of RBCs during an open heart surgery 6 months ago. His antibody screen was negative at that time. He has returned for a follow up surgery and his antibody screen is now positive with both screen cells at the AHG phase.



Reactions are occurring at AHG phase which indicates a possible clinically significant antibody, Jka showing dosage.

Refer to Case Study 1 panel below to see reactions of antibody panel.

IS = Immediate Spin; AHG = Antihuman Globulin Phase; CC = Check Cells; AC = Auto Control; ND= Not done

Case study 1 conclusion:

Patient's previous transfusion 6 months ago exposed him to the Jka antigen, causing the formation of this antibody which is known for showing dosage.


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