These methodologies use principles that amplify or multiply the target of interest, usually incorporating an enzyme to produce millions or billions of copies in a relatively short time.

Some enzymes used in amplification include:

• DNA ligase

• DNA polymerase

• RNA polymerase

• Reverse transcriptase

• Alkaline phosphatase

• Cleavase

Note: the steps in amplified testing will vary depending on the target amplified, requirement for thermal cycling and detection techniques.