Specimen Collection & Handling
Specimen Collection and Handling
Some global specimen collection and handling issues to consider include:
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Specimens that contain nucleated cells will be of interest in DNA methodologies while specimens lacking nucleated cells are more useful in RNA methodologies. -
rRNA is more stable than mRNA, which is labile and sensitive to contamination. -
DNA is relatively stable and can be obtained from nonviable sources. -
Serum or plasma obtained by standard routine venipuncture procedures can be used as long as proper site selection and decontamination occur. -
Standard anticoagulants such as Ethylenediaminetetraacetic Acid (EDTA) and Acid Citrate Dextrose (ACD) can be used; however avoid the use of heparin as an anticoagulant as it interferes with some polymerase chain reaction (PCR) methodologies. -
When using fluorescence, fasting serum or whole blood specimens should be used to decrease the interference by lipids.
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