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Review 1
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The page below is a sample from the LabCE course
Case Studies in Clinical Microbiology
. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.
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Review 1
Smith KR, Fisher HC III, Hook, EW III: Prevalence of fluorescent monoclonal antibody-nonreactive
Neisseria gonorrhoeae
in five North American sexually transmitted disease clinics.
J Clin Microbiol 34:1551-1552, 1996
We compared a direct fluorescent monoclonal antibody (DFA) test with alternative enzymatic and fermention tests for identifying presumptive gonococcal isolates in a systematic sample from patients attending five sexually transmitted disease clinics in five cities.
Fourteen (2.5%) of 556 isolates from three clinics were nonreactive with the DFA confirmatory reagent and reactive by both the Quad-Ferm and Rapid NH tests. The prevalence of DFA-nonreactive
Neisseria gonorrhoeae
isolates varies geographically and is independent of local methods for the identification of possible gonococci.
On the basis of our findings, we recommend that for use in medicolegal and other instances in which a diagnosis of gonorrhea has the potential to have far-reaching effects, it is appropriate to test DFA reagent-nonreactive, oxidase-positive, gram-negative diplococci by alternative methods of gonococcal confirmation.
Although the prevalence of such isolates could change, the fluorescent monoclonal antibody confirmation reagents remain useful for many clinical situations. Their ease of use and ready applicability for screening large numbers of isolates make them useful for many laboratories.
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