The test for glucose is a double sequential enzyme reaction, utilizing the glucose-oxidase/peroxidase method. In the first reaction, glucose oxidase catalyzes the oxidation of glucose to gluconic acid and hydrogen peroxide. Then, the peroxidase catalyzes the oxidation of a chromogen by the hydrogen peroxide to form a colored product. This method does not react with lactose, fructose or galactose. Study the dipstick color chart to become familiar with the range of color changes. The urine specimen should be at room temperature for these enzyme reactions to occur properly.
