Basis of the Urine pH Test

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The page below is a sample from the LabCE course Chemical Screening of Urine by Reagent Strip (retired March 2012). Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Basis of the Urine pH Test

Dipsticks measure pH using methyl red and bromthymol blue indicator dyes. The color change that occurs in this test area correlates with the urine's pH. Sensitivity to pH ranges from 5.0 (acid pH) to 9.0 (alkaline pH) on a urine reagent strip. Physiological urine pH ranges from 5.0 to 8.0.