Background and Introduction to the ABO System

Table 3: Testing the Serum with Known Red Cells (Reverse Typing)

It has been demonstrated that antibodies occur predictably in the sera of all normal adults in association with the ABO antigens. Demonstration of these antibodies is therefore necessary for definitive classification of an individual’s ABO cell type. The individual’s serum is therefore tested against reagent red cells containing known antigens.

Patient ABO Blood Group

Patient Serum Tested with Known Reagent Cells

A Cells

B Cells

A

0

4+

B

4+

0

O

4+

4+

AB

0

0

+ = agglutination (graded 1+ to 4+)
0 = no agglutination or hemolysis

Earn ASCLS P.A.C.E.® credits for your AMT, ASCP, or NCA recertification requirements.

Introduction to the ABO Blood Group System course details »

Learn more about medical technologist continuing education for MTs, MLTs, and other lab personnel »

Get information on laboratory compliance training for clinical and medical laboratories »