Sodium Information and Courses from MediaLab, Inc.

The following table lists some of the chemicals present in CSF, and their concentrations: ChemicalLevel sodium 136.0 - 150.0 m Eq/L potassium 2.3 - 2.7 m Eq/L magnesium2.4 - 3.0 m Eq/Lprotein2 - 4 mg/dL (normally diffuses across blood-brain barrier) glucose 45.0 - 60.0 mg/dL calcium2.1 - 2.7 m Eq/dLcholesterolpresent in small amounts creatinine 0.5 - 1.2 mg/dL lactic acid dehyrdogenase (LDH) present in small amounts phosphorus (inorganic)1.0 - 2.0 mg/dLurea6.0 - 16.0 mg/dL uric acid 0.5 - 3.0 mg/dL

Testing for ketone bodies is based on a nitroprusside reaction. Acetoacetic acid reacts with sodium nitroferricyanide and glycerine in an alkaline medium to produce a violet-to-purple colored complex. The reagent strip method can detect as little as 5 mg/dL acetoacetic acid in urine. It does not react with acetone unless glycine is present or B-hydroxybutyric acid. Since these two compounds are derived from acetoacetic acid, their presence can be assumed if the test for ketones is positive. Ketones are reported either as negative, small, moderate or large amounts; or negative, 1+, 2+, 3+, or 4+. In some severe cases of ketosis, it may be necessary to perform tests on serial dilutions to provide more information on the quantity of ketones present.

There are several acids which can be used to precipitate proteins - sulfosalicylic, trichloroacetic, nitric, and acetic acids. Sulfosalicylic acid (SSA) is the most frequently used acid test because it does not require the use of heat. Exton’s reagent is 5% sulfosalicylic acid in a solution of sodium sulfate. Exton (1925) found that adding sodium sulfate to the SSA causes a more uniform precipitate to be formed. To perform the SSA procedure mix equal parts of patient urine and the reagent. Rate the amount of turbidity according to the following scale:

Denaturing chemicals can be added to the acrylamides during formation of polyacrylamide gels. These additives keep the solutes or molecules in a denatured state during separation. Urea denatures double-stranded DNA to single-stranded DNA. A detergent, sodium dodecyl sulfate (SDS), denatures proteins. Adding SDS with heat denatures proteins to small, similar shaped particles and coats each so that protein structures are not reformed. SDS is usually added to the gel and the protein sample. Then the mixture of protein coated fragments moves through polyacrylamide gel pores with speed similar to a mixture of DNA fragments.

The specimen of choice for coagulation testing is plasma. Venous blood is drawn into a 3.2% buffered sodium citrate tube (blue top tube), yielding a whole blood sample with a 9:1 blood to anticoagulant ratio. Inadequate filling of the collection tube will decrease this ratio, and may affect test results. A blue top tube used for coagulation testing should be drawn before any other tubes containing additives. This includes tubes containing other anticoagulants and/or plastic serum tubes containing clot activators. A serum tube that does not contain an additive can be collected before the blue top tube. If a winged blood collection set is used in drawing a specimen for coagulation testing, a discard tube should be drawn first. The discard tube must be used to fill the blood collection tubing dead space to assure that the proper anticoagulant/blood ratio is maintained, but the discard tube does not need to be completely filled. The discard tube should be a nonadditive or a coagulation tube. If a blood specimen used for coagulation testing must be collected from an indwelling line that may contain heparin, the line should be flushed with 5 mL of saline, and the first 5 mL of blood or 6-times the line volume (dead space volume of the catheter) be drawn off and discarded before the coagulation tube is filled.

Blood is tested for the most important electrolytes (salts): Sodium (Na) Potassium (K) Chloride (Cl) Carbon dioxide (CO2)Can be run on serum or plasma.

These tubes contain the anticoagulant sodium citrate. They are used mostly for coagulation (clotting) studies. They must be completely filled to assure proper ratio of anticoagulant to blood.They must be inverted immediately after filling to prevent clotting.

Contain either sodium heparin or sodium EDTA anticoagulants, or no anticoagulant. Are used for trace element, toxicology, and nutritional studies.

Following liquefaction (20-30 minutes), mix the sample manually by swirling the container several times. Thorough mixing is essential for accurate counting. Calibrated automatic pipettes are used to prepare a dilution. Because of the viscosity of semen, the semen should be added to the diluent using a positive pressure pipettor. The dilution often used for routine sperm counts is 1:20 but the actual dilution factor will vary depending on the total sperm count. For high concentration specimens a greater dilution will be necessary. For low concentrations an undiluted or minimally diluted specimen may be required. The appropriate dilution is determined by estimating the concentration needed to do a count of at least 100 cells per side of the loaded hemacytometer. The diluent that may be used for sperm counts on a hemacytometer can be as follows: 5 gm of sodium bicarbonate in 100 ml of distilled water, plus 1ml of formalin (neutral).

Amorphous urates can be dissolved in dilute sodium hydroxide. Amorphous phosphates will dissolve in dilute acetic acid. In either case, the bacteria will remain.