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Serological Information and Courses from MediaLab, Inc.

These are the MediaLab courses that cover Serological and links to relevant pages within the course.

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Laboratories Individuals

Cerebrospinal Fluid
Collection Tubes (continued)

The samples from each tube are used for specific tests:The first tube may also be used for serological testing.The second tube is used for gram stain and culture.The third tube is used for the cell count and differential.The fourth tube is used for cytological examinations or other tests which may be needed to further characterize abnormal cells.

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CLIA Blood Banking Review
Which of the following tests must be repeated by the lab on homologous blood received from the Red Cross or other community blood sources:View Page

CLIA General Laboratory Review
A definitive diagnosis of malaria can be made by:View Page
Which of the following would be considered most significant as it relates to serological testing:View Page
Match these autoimmune diseases with their corresponding serological markers:View Page

CLIA Microbiology / Serology Review
The Quelling test is useful for which of the following :View Page
Which of the following best describes the benefits of the RPR or VDRL tests:View Page
Which of the following serological tests would be used for the diagnosis of Q-fever:View Page
Which of the following two are useful serological tests to document antecedent Streptococcal infections:View Page

Current Topics in Clinical Microbiology
Beta hemolytic colonies grew from the blood culture bottle after 18 hours incubation (see photograph). The following tests would be helpful in making a preliminary identification:View Page

Introduction to the ABO Blood Group System
Deducing the Gene

The presence of A and/or B antigen on the red cells can be recognized by serological tests with the appropriate antisera so that the presence of the gene that controls its production can be deduced in the absence of both A and B genes (when no A or B antigen is present on the red cells).

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Semen Analysis
Materials Needed

The following is a list of materials needed for semen analysis. Laboratories will differ slightly in the equipment used. Use of this equipment will be described further in the later pages of this course. Materials needed include:graduated test tube or serological pipets with safety bulb to measure volumepH paper in neutral to basic range (e.g. 7.2-8.8)counting chamber and/or automated counting machineglass slides and coverslips for wet mount if motility and sperm count are to be assessed separatelyhand counterif dilution is donediluting fluid calibrated automatic pipetspositive pressure pipets and glass boreslight microscope with phase contrast objectives for sperm count and bright field objectives for morphology assessmentglass slides and fixative for morphology slidesset-up for performing Papanicolaou or other morphology stainingEvery laboratory should also have a copy of the "WHO Laboratory Manual for the Examination of Human Semen and Sperm-Cervical Mucus Interaction", published on behalf of the WHO by Cambridge University Press. The fourth edition was published in 1999.

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In summary the procedure will involve the following:

The following aspects of semen analysis will be described in further detail during this course: Check the identity of the patient Record information that has been obtained from the patient including: time of collection, collection method, problems during collection, medications the patient is taking Note time to liquefaction Measure the volume by pouring into a graduated test tube or by drawing the specimen into an appropriately sized graduated serological pipet Assess viscosity Note color Measure pH by putting a drop on a strip of pH paper Count the sperm in the specimen Assess motility Count round cells, if present Assess the proportion of round cells that are white cells Fix and prepare specimen for morphology assessment; assess morphology

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