Saline Information and Courses from MediaLab, Inc.
These are the MediaLab courses that cover Saline and links to relevant pages within the course.
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| Diluting Fluids There are several diluents that may be used for CSF counts. Normal saline should be used to make dilutions for total cell counts. Diluting fluids for WBC counts include:crystal violet/acetic acidgentian violet/acetic acidtoluidine blue 0 and saponinThese fluids stain the white cells and lyse the red cells. The red cell count can be obtained by subtracting the white cell count from the total count.
Low power (10x) may be used for the total count while the high power objective (40x) is suggested for the white cell count, especially if the white cells are to be differentiated into segs, lymphs and monocytes.
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| Which of the following diluting fluids are suitable for spinal fluid counts? | View Page |
| Which of the following antibodies is detected primarily in the antiglobulin phase of the crossmatch: | View Page |
| Rh antibodies generally: | View Page |
| A false-negative reaction while performing the DAT technique may be the result of: | View Page |
| The process of pipetting 1.0 ml of plasma or serum into a tube containing 1.0 ml of saline, mixing the contents and then repeating the same procedure into several additional tubes also containing 1.0 ml of saline is referred to as: | View Page |
| The concentration of sodium chloride in an isotonic solution is : | View Page |
| Which of the following is used as the indicator in the rapid carbohydrate utilization tests: | View Page |
| The Iodine prep method is used to detect which of the following protozoan stages: | View Page |
| Collecting Blood Specimens for Coagulation Testing The specimen of choice for coagulation testing is plasma. Venous blood is drawn into a 3.2% buffered sodium citrate tube (blue top tube), yielding a whole blood sample with a 9:1 blood to anticoagulant ratio. Inadequate filling of the collection tube will decrease this ratio, and may affect test results. A blue top tube used for coagulation testing should be drawn before any other tubes containing additives. This includes tubes containing other anticoagulants and/or plastic serum tubes containing clot activators. A serum tube that does not contain an additive can be collected before the blue top tube. If a winged blood collection set is used in drawing a specimen for coagulation testing, a discard tube should be drawn first. The discard tube must be used to fill the blood collection tubing dead space to assure that the proper anticoagulant/blood ratio is maintained, but the discard tube does not need to be completely filled. The discard tube should be a nonadditive or a coagulation tube. If a blood specimen used for coagulation testing must be collected from an indwelling line that may contain heparin, the line should be flushed with 5 mL of saline, and the first 5 mL of blood or 6-times the line volume (dead space volume of the catheter) be drawn off and discarded before the coagulation tube is filled. | View Page |
| If an Exposure Occurs Give first aid. Wash needlesticks and cuts with soap and water. Flush splashes to the nose, mouth, or skin with water. Irrigate eyes with clean water, saline, or sterile irrigants. Report exposure to supervisor. | View Page |
| The dematiaceous conidium illustrated in this photomicrograph was obtained from a tiny portion of dark colony that grew to maturity in six days. Spores incubated in a saline mount for four hours developed germ tubes from both terminal cells. The features observed confirm the identification of: | View Page |
| Rouleaux Rouleaux formation correlates with an increased concentration of serum monoclonal proteins. Rouleaux may be seen as an artifact in the thicker portions of blood smears. The addition of a drop of saline to the blood smear will serve to disperse any artifactual rouleaux formation. The presence of rouleaux formation or RBC agglutination may result in a falsely decreased electronic red blood count and falsely increased MCV, as these clusters may be read as one cell. | View Page |