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Online compliance and continuing education courses for clinical laboratories

Safety Information and Courses from MediaLab, Inc.

These are the MediaLab courses that cover Safety and links to relevant pages within the course.

Learn more about laboratory continuing education for medical technologists to earn CE credit for AMT, ASCP, NCA, and state license renewal and recertification. Or get information about laboratory safety and compliance courses that deliver cost-effective OSHA safety training and continuing education to your laboratory's employees.

Laboratories Individuals

Cerebrospinal Fluid
Safety Precautions

Important safety precautions must be observed when handling cerebrospinal fluid. The following guidelines apply:Semi-automatic micropipettes and disposable plastic chambers are the safest option for CSF testing. Many laboratories still use the hemacytometer with disposable pipets.If disposable materials are not used, soak contaminated reusable pipets, hemacytometer and coverslip in 70% alcohol or Wexide.All disposable items should be placed in a biohazard container for appropriate disposal.Wash hands thoroughly when the examination is completed.Spinal fluids which are to be discarded must be placed in biohazard containers for appropriate disposal.Careful attention to specimen processing and handling will help ensure that accurate results are obtained.

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CLIA Blood Banking Review
Which of the following activities will put an employee at risk for exposure to a Bloodborne Pathogen (BBP)?View Page
What type of Personal Protective Equipment (PPE) is necessary when opening a centrifuge (chance for splashing)?View Page

CLIA General Laboratory Review
What type of protective gear must be worn as a minimum when working with hazardous chemicals:View Page

Department of Transportation (DOT) Regulated Urine Specimen Collection Training
Federal drug testing custody and control form (CCF)

The federal drug testing custody and control form (CCF) must be used to document every urine collection required by the Department of Transportation drug testing program. At the present time, these include the: Federal Motor Carrier Safety Administration (FMCSA) Federal Aviation Administration (FAA) Research and Special Programs Administration (Pipeline) (RSPA) Federal Transit Administration (FTA) Federal Railroad Administration (FRA) United States Coast Guard (USCG)

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Exceptions

It should also be understood that a Federally Regulated CCF need not be used in every situation involving a company regulated by the Department of Transportation. For example, if an interstate truck driver slips and falls while standing on the loading dock of a trucking terminal, and the trucking company for which the driver works requires a post-accident drug screen, a Non-Federally Regulated CCF would be appropriate for this collection. The driver was not involved in a safety sensitive assignment. On the other hand, if the driver had an accident while driving, then a Federally Regulated CCF must be used since the driver was in a safety sensitive position when the accident occurred.

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Unacceptable forms of identification

Unacceptable forms of identification include: Identification by a co-worker. Identification by another employee also working in a safety sensitive position. Use of a single non-photo identification card such as a social security card, credit card, membership card, pay voucher, or voter registration card. Faxed or photocopies of identification documents.

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First Aid
Introduction

An important component of safety training is a working knowledge of first aid and the medical services available to you.This program will explain several common injuries and their treatment.

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Treatment of Chemical Burns of Eye

Keep the affected eye open using your fingers. Immediately begin flushing the eyes with water and continue for 15 minutes. Use an eyewash, safety shower, or water from the sink.Assist the victim by supporting the head so that water flows across the eyeball from the inside corner of the eye (nearest the nose), outward. This will prevent chemical from getting into the unaffected eye.Get immediate medical help.

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HIV Safety for Florida
Which of the following properly describes use of needles?View Page
Sharps

A sharp is any object which can penetrate the skin, including: needles scalpels broken glassPlace all needles and other sharps in puncture resistant sharps disposal containers as soon as possible after use.Properly use the safety devices provided with needles.

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Face and Eye Protection

Face shields, masks, and safety glasses protect your eyes and the mucous membranes of your nose and mouth.They must be worn whenever it is reasonably anticipated that splashing or spraying of blood or other contaminated materials may occur.Employees who wear prescription eyewear may be protected with a face shield, goggles, or with side shields attached to their glasses.

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Introduction to Bioterrorism
Laboratory Response

The broad base of clinical laboratories in this country is an essential component of our nation’s public health and healthcare system and is an essential link in addressing biological and chemical terrorism. In 1999 the Centers for Disease Control and Prevention (CDC) initiated the concept of a Laboratory Response Network (LRN).  The LRN is a network of local, state, federal, and military laboratories across the United States and internationally which work together in an integrated and coordinated way for a rapid response to public health emergencies. The LRN concept of operations is based on a system of safety and proficiency.

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Sentinel Labs

The frontline clinical microbiology laboratories are known as “sentinel laboratories”. The sentinel laboratories play a key role in the nation’s preparedness efforts. These laboratories perform the initial screening of clinical specimens for potential pathogens (rule-out) and refer specimens or isolates to a state or local public health laboratory at the reference level of the LRN. There are two kinds of sentinel laboratories: advanced and basic. Classification depends on their biological safety level and analytical capability.

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Your Response – At Work

Recent events, including the terrorist attacks on September 11, 2001 and the subsequent bioterrorist releases of anthrax, have been a harsh awakening that the nation’s workplaces could be terrorist targets.Traditionally laboratory safety guidelines have emphasized use of optimal work practices, appropriate containment equipment, well-designed facilities, and administrative controls to minimize risks of unintentional infection or injury for laboratory workers. Today, in addition to the above, laboratories must make a risk and threat assessment, secure data and electronic technology systems, plus develop policies regarding specimen accountability, facility security, and emergency response.The next few pages will cover a number of things that you can do to assist in making your laboratory more risk free to a terrorist attack and some things you can do in case that security is breached. You too have a role in the security of your workplace!

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Laws and Rules of the Florida Board of Clinical Laboratory Personnel
Capability Violations

The accuracy and safety of patient testing depends on the capability and honesty of clinical laboratory personnel. If an individual's ability to perform testing is influenced by illness, injury, drug use (legal and illegal), or alcohol use, he or she may no longer practice. The Board can order a doctor's exam to determine if illness, injury, drugs, or alcohol is a factor. The individual can get his / her license back after recovery and proving that the condition is no longer a problem. If an individual commits a crime in any state relating to matters of honesty (such as filing false reports or advertising false services), that individual's Florida license may be suspended. Other licensed personnel who know that an individual is practicing despite being under the influence of drugs or alcohol, is physically or mentally incapable, has been convicted of a lab-related crime, or is not competent to perform his / her duties are required to report the individual to the Board. The following are violations of Board rules:Continuing to practice after becoming unable to safely perform testing because of illness or use of alcohol or drugs, or another mental or physical condition. Continuing to practice after being judged mentally or physically incapable.Being convicted of any crime relating to activities of clinical laboratory science or involving dishonesty or lack of morals. Failing to report to the Board that one has been convicted of a crime (as listed above), been judged mentally or physically incapable, or had a licensed revoked in another state. Knowingly allow an unqualified person to perform clinical laboratory duties.

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Medical Error Prevention
The Joint Commission on Accreditation of Healthcare Organizations promotes improved patient safety through its programs and resources. True or false?View Page
These statements describe things related to medical error prevention.View Page
Root Cause Analysis

JCAHO has a very important role in cause analysis of medical errors. Since 1995, it has been increasing its focus on patient safety by requiring in-depth analysis, Root Cause Analysis, to determine the underlying causes of every adverse event. Root causes are specific reasons that contribute to medical errors. They cause mistakes that lead to great patient harm (adverse events). Usually they can be identified. Examples: Using a wrong calculation factor Neglecting to use directions for complicated tests Reporting the wrong test result Using outdated reagents Testing clotted or partially-filled samplesDiluting a test sample incorrectly In most cases, management has the authority and means to resolve root causes. Root Cause Analysis also recommends actions to prevent reoccurrence of an adverse event.

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Study

The Internet provides extensive, current resources for studying ways to prevent errors. Performing word searches for Medical Errors or Patient Safety or Laboratory Errors identifies a wealth of Internet resources. A Patient Safety search lists more than 93 million items.

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New JCAHO Standards

The healthcare community uses RCA to reduce medical errors, but it is reactive in nature. For this reason, JCAHO collaborates with recognized patient safety experts to develop and implement additional patient safety standards. These new standards charge healthcare organization leaders to create cultures of patient safety. They emphasize the need for teamwork and effective communication. They are based on well-known experiences of the aviation industry and they reflect findings from JCAHO's Sentinel Event Database. They identify communication breakdowns as the most common underlying factor in all types of Sentinel Events.

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JCAHO Patient Safety Goals JCAHO adopted national patient safety goals for laboratories and many other healthcare organizations. 2006 Laboratory Services National Patient Safety Goals These goals are directly quoted.View Page
Choose the organization(s) that strive to improve patient safety.View Page
Improving Patient Safety Many other organizations strive to improve patient safety. These dedicated groups promote current information about their goals, projects, progress, publications, tools, and educational offerings in their Internet Websites.View Page
American Society for Clinical Pathology The American Society for Clinical Pathology, ASCP, promotes medical error prevention through its projects, programs, and activities. It includes this important topic in its many 400 workshops, symposia, teleconferences, and self-study programs. ASCP also promotes error prevention in the medical textbooks, reference manuals, slide atlases, audiovisual materials, and computer software it publishes. Its membership newsletters and The American Journal of Clinical Pathology and LabMedicine journal frequently address error prevention and patient safety.View Page
American Society for Clinical Laboratory ScienceThe American Society for Clinical Laboratory Science, ASCLS, joins the leadership effort to prevent medical errors and increase patient safety.View Page
Public Responsibility for Safety

People can help prevent errors in their medical care by understanding their treatment, keeping organized health records, and asking questions. They should feel comfortable talking with medical professionals when things do not seem right.

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Factors that Contribute to Medical ErrorsView Page

OSHA Bloodborne Pathogens
Engineering Controls

Engineering Controls are devices which isolate the worker from the hazard of exposure.Examples: Self-sheathing needles Sharps disposal containers Disposable resuscitation bags Microbiological safety cabinets Proper use of engineering controls in your workplace will help protect you from bloodborne pathogens.

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Face and Eye Protection

The following protect your eyes and the mucous membranes of your nose and mouth: Face shields Masks and safety glasses They must be worn whenever it is reasonably anticipated that splashing or spraying of blood or other contaminated materials may occur.Employees who wear prescription eyewear may be protected with a face shield, goggles, or with side shields attached to their glasses.

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Methods of Control

Methods of control are ways you can protect yourself from bloodborne pathogens using proper equipment, safety features, and work practices. The next few pages will acquaint you with these ways to keep yourself safe.

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OSHA Chemical Hygiene (updated 2007)
Employer Responsibility

Your employer has a responsibility to educate you about chemical hazards, and safety procedures.

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Your Responsibility

Read the manufacturers' labels and MSDS sheets and follow the instructions and warnings. Access pertinent safety information through your supervisor. If you detect any potential hazards either in the facility or in your work procedures, contact your supervisor as soon as possible.

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Handling and Storage Data

Information concerning the eye, skin, and respiratory protection required while using the chemical. Safety goggles are the minimum eye protection and rubber or nitrile gloves must be worn when handling any chemical. Any special ventilation that might be needed.

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General Laboratory Precautions

Laboratory safety includes a number of precautions designed to protect you and your coworkers. Remember that: eating drinking smoking applying cosmetics or lip balm are forbidden in areas where chemicals are present.

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Personal Protective Equipment

Personal protective equipment is an essential way to protect yourself from the dangers of chemicals. You'll find on the label or MSDS exactly what kinds of clothing, gloves, and coverings you'll need to keep yourself safe. Also, the laboratory's chemical hygiene plan will include information about necessary personal protective equipment and engineering controls that will reduce your exposure to hazardous chemicals. At a minimum, safety goggles and rubber or nitrile gloves (not necessarily utility gloves) are necessary parts of your personal protective equipment.

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Monitoring

Certain chemicals in use in the laboratory, such as formaldehyde, are hazardous if your exposure to them is too prolonged. The amount of the chemical to which you can be exposed before possible danger is called the threshold limit value. Monitoring badges are used from time to time to measure your exposure. These are worn in the "breathing zone" for a certain period of time--often eight hours (for long-term exposure) or fifteen minutes (for short-term exposure). Based on the results of this monitoring, additional personal safety measures, such as ventilation or face-fitted masks, may be implemented for your protection.

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OSHA Electrical Safety (updated 2007)
Space heaters

Electrical space heaters are prohibited unless they are approved for use and inspected by your facilities management department. Space heaters are not permitted in hospital sleeping areas or in laboratories containing flammable liquids or gases. The heating elements in the space heater cannot exceed 212 degrees Fahrenheit, and cannot be exposed elements. Heaters with hot elements, even with guards, are not allowed. The approved heater must be connected directly to an outlet without the use of an extension cord. Reference: NFPA 101 Life Safety Code. National Fire Protection Agency. Paragraph 18/19.7.8. Quincy, MA: 2006.

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Electrical Hazard Awareness

Manufacturers are required to label appliances and instruments with electrical ratings including voltage, frequency, current, and/or wattage of the device and precautionary statements if applicable. Operating and safety instructions are provided with electrical equipment. It is prudent for personnel to familiarize themselves with this information before using the equipment. Personnel should be aware of the hazards associated with the use of defective electrical equipment. Defective equipment should be tagged and repaired or discarded. Keep liquids, chemicals, and heat sources away from electrical outlets and cords.

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OSHA Formaldehyde
A Potential Carcinogen

More importantly, the National Institute for Occupational Safety and Health (NIOSH) has labeled formaldehyde a potential human carcinogen.

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Acceptable Results

If the monitoring results are below the action level, no additional monitoring is required unless there is a change in procedure.However, annual monitoring is recommended as a good safety practice.

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PPE

When using formaldehyde in any concentration, with the exception of putting specimens in single vials, you must wear: A cover gown or apron A face shield or safety goggles Gloves This personal protective equipment is provided at no cost to you.

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Safety is no accident!

These rules and regulations are designed to protect YOU--our most valuable resource!

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Phlebotomy
Case

Julie Smith, a newly certified phlebotomist at Northlake Hospital, entered a patient’s room on the third floor for a routine blood draw. The patient was an elderly woman who had very small fragile veins. Julie therefore decided to use a safety butterfly needle attached to a Vacutainer tube in order to draw the blood. When Julie was finished with the venipuncture, she detached the butterfly needle from the Vacutainer, and approached the Biohazard needle disposal box. She noticed that the disposal box was full , but decided to try to fit the butterfly into the box anyway. Holding the butterfly by the tubing, she tried to push the butterfly into the box. The needle suddenly recoiled and stuck Julie’s finger. Julie left the patient’s room in a panic and headed back to the lab to report the needle stick injury.

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What should Julie have done to prevent the needle stick?View Page
Discussion

All biohazard needle disposal containers are marked with a “full” line at about ¾ of the box’s volume. Therefore, needles should never be sticking out of the top of the container. Julie should never have attempted to put the needle into an overly full container. The needlestick safety and prevention act requires the use of butterflies with built in safety devices. However, they are only effective if properly activated. When disposing of a butterfly needle, hold its “wings” with one hand, and the hub at the opposite end of the tubing with your other hand to prevent the needle from recoiling. Butterflies should be used with extra caution since they are the number one cause of needle stick injuries.Relevant topics:Needle disposal, Sharps disposal containers, Butterfly needles with safety 1, Butterfly needles with safety 2, Needle-stick injuries, Built-in safety features, Angel Wing™ safety butterfly, Punctur-Guard™ safety butterfly

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Case

A phlebotomist at Memorial Hills Hospital entered the room of a 6 year old patient. The only test ordered was a CBC, so the phlebotomist decided to do a finger stick. After gathering proper supplies for the finger stick, the phlebotomist began the procedure by putting on gloves and wiping the tip and side of the patient’s ring finger with alcohol. He positioned the safety lancet between the ball and the side of the finger and made a small incision. The child cried as the blood was collected.

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Butterfly needles with built-in safety features continued

Two examples of butterfly needles with built-in safety devices are shown.The Punctur-Guard™ (Bioplexus), shown above, uses an internal blunt needle which is activated after blood is drawn. The activated device showing the blunt internal needle is shown in the inset on the upper right. The Angel Wing ™ (Monoject), is activated by sliding a safety shield over the needle after venipuncture.

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Needle holders with built-in protection devices

You will be required to use appropriate safety devices with your needle holder. Various options are available. The Needle-Pro™ Needle protection device (Sims Portex) shown here is an example of a needle holder with a built-in safety device. It is activated after venipuncture by pressing the orange protection device against any flat surface so as to engage the sheath firmly on the needle.

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Multiple draw needles with built-in safety features.

You will be required to use multiple draw needles with built-in Safety features. One example is the Puncture-Guard™ (BioPlexus) needle, which uses an internal blunt needle (detail above) that is activated with forward pressure on the final blood tube prior to withdrawal of the needle from the vein. Refer to your institution’s and the manufacturer’s procedure manuals before using these devices.

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Needle holders, built-in protection devices contd

When the orange safety sheath is engaged, as shown on the right, it protects the user from needlestick injury, until the device is properly disposed of in an approved sharps container. Refer to the manufacturer’s instructions, and your procedure manual for detailed instructions prior to use.

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Syringes with built-in safety devices

Syringes are used for injections, as well as to collect blood. There a various syringes with built-in safety features.One example is the Monoject™ (Sherwood Services AG), Safety Syringe, shown here.

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Syringes with built-in safety devices contd.

After use, a safety shield is slid over the needle, and locked into place. The safety syringe with the shield locked in place is shown here.

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Needles with built-in safety devices

You will be using needles and/or needle holders with built-in safety devices. Various such needles are on the market. Remember that you must still activate the safety device to get the protection offered. So careful attention to what you are doing is still one of the best ways to protect yourself against needlestick injury.

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Butterflies with built-in safety features

You will be using butterfly needles with built in safety features. Butterfly needles are the number-one cause of needlestick injuries, so proper use of their safety devices is critical. Their use is described in greater detail in the section on butterfly needle blood collection.

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Activation of safety device

Activate the safety device in use at your institution.Be sure to follow your institution’s procedure for activating this device to protect yourself from needlestick exposure.

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Butterfly needle - Angel WingTM safety butterfly

The Angel Wing™ (Monoject), is activated by sliding a stainless steel safety shield over the needle after withdrawal from the vein.The Angel Wing™ (Monoject), is activated by sliding a stainless steel safety shield over the needle after withdrawal from the vein.

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Butterfly needle - Butterfly needles with built-in safety features

You will be using butterfly needles with built-in safety device. The safety device must be activated upon completion of the blood collection.You will be using butterfly needles with built-in safety device. The safety device must be activated upon completion of the blood collection.The Angel Wing™ (Monoject) safety butterfly is shown here.

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Finger stick - Finger stick collections

A finger-stick collection is performed by piercing the fingertip with a safety Lancet, which controls the depth of incision, and collecting capillary blood. The BD Microtainer™ Brand Safety Flow Lancet is shown here.Finger-sticks should not be performed on children under one year of age.

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Finger stick - puncture

Select a safety lancet appropriate for the size of the patient’s finger.You may warm the finger prior to puncture to increase blood flow.Make the puncture perpendicular, rather than parallel, to the finger print.

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Syringe - Transferring blood to collection tubes

After collecting the blood specimen into a syringe, properly activate the appropriate safety device, and dispose of the needle in a sharps container.Attach the syringe to a blood transfer device by twisting the needle tip into the hub of the device.Push a vacuum blood collection tube into the holder of the transfer device, and let the tube fill to the appropriate level.

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Butterfly needle - Punctur-GuardTM safety butterfly

Another type of safety butterfly needle is shown to the right.The Punctur-Guard™ (Bioplexus) uses an internal blunt needle (arrow) that is activated by manually locking a small lever on the butterfly. Please refer to your facility’s and the manufacturer’s procedure manuals for detailed instructions.

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Activate needle safety device

After collecting the blood, activate the needle safety device according to manufacturer’s instructions, and place it in a sharps disposal container. If blood was collected into a syringe, insert the syringe tip into the hub of a blood transfer device, and rotate the syringe clockwise to secure it to the device. Push the blood culture bottle into the holder of the transfer device, and draw the appropriate volume of blood into the blood culture bottles.

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Needlestick safety and prevention act continued

Please keep the following in mind: These devices must be used properly and conscientiously to prevent injury. Carefully follow your institution’s policies and procedures to prevent needlestick injury. Most devices require you to activate their safety features. Often, the device lets you know it is properly activated by a click or a snap.

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OSHA bloodborne pathogens standard

The Occupational Health and Safety Administration (OSHA), of the federal government has mandated bloodborne pathogen training for all US workers who are at risk of exposure. The next few slides cover a few highlights of this training. You will receive complete OSHA bloodborne pathogens training before you begin work.

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Needlestick safety and prevention act

The Needlestick Safety and Prevention Act was passed by Congress in November 2000. This law modifies the OSHA Bloodborne Pathogens Standard to require that health-care institutions use only needles and other sharps which have engineering controls and design features to help prevent accidental sharps injury. Health care institutions must update their exposure control plans to reflect these changes.

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Needlestick safety and prevention act continued

There are many different types of needle safety devices on the market. As you go through this program, we will introduce you to some of them

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Needlestick safety and prevention act continued

The law requires that each institution gets input from employees actually involved in blood collection. So the actual safety devices you are required to use will vary depending on where you work.

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Quality Control
Safety and Handling of Controls

To ensure the safety of those performing patient testing, controls do not contain HIV or the hepatitis B virus. Manufacturers place the same batch of control material into small vials. This allows only a small portion of the control to be handled while the remainder is stored until needed. Storage information for controls is printed on the label. These instructions should be followed carefully in order to prevent contamination or false results.

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Semen Analysis
Materials Needed

The following is a list of materials needed for semen analysis. Laboratories will differ slightly in the equipment used. Use of this equipment will be described further in the later pages of this course. Materials needed include:graduated test tube or serological pipets with safety bulb to measure volumepH paper in neutral to basic range (e.g. 7.2-8.8)counting chamber and/or automated counting machineglass slides and coverslips for wet mount if motility and sperm count are to be assessed separatelyhand counterif dilution is donediluting fluid calibrated automatic pipetspositive pressure pipets and glass boreslight microscope with phase contrast objectives for sperm count and bright field objectives for morphology assessmentglass slides and fixative for morphology slidesset-up for performing Papanicolaou or other morphology stainingEvery laboratory should also have a copy of the "WHO Laboratory Manual for the Examination of Human Semen and Sperm-Cervical Mucus Interaction", published on behalf of the WHO by Cambridge University Press. The fourth edition was published in 1999.

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Safety Precautions

Safety precautions should be observed when handling seminal fluid. The following guidelines should be followed:If non-disposable items are used, soak contaminated items(e.g.hemacytometers and coverslips) in 70% alcohol or Wexide®.All disposable items should be placed in a biohazard bag for autoclaving.Gloves must be worn and hands thoroughly washed when the examination is completed.Seminal fluids that are to be discarded should be placed in biohazard bags for autoclaving.

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Tuberculosis Awareness for Healthcare Workers
TB Infection Control in the Laboratory

The laboratory director is responsible for the development of a risk-based infection control plan for the laboratory.The personnel are trained in methods that minimize the production of aerosols.A respirator is used when performing procedures that can result in aerosolization outside a biological safety cabinet.Personal protective equipment specified in the infection control plan is used.Disposable gloves are worn for all laboratory procedures.

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Biosafety Levels

Laboratory workers who handle infectious materials in the microbiology laboratory should be aware of the work practices, safety equipment, and barriers that will protect them and others in the area from infectious agents. The Centers for Disease Control and Prevention (CDC) and the National Institutes of Health (NIH) created guidelines to assist laboratories in developing safe practices based on the infectious agents that are handled. These guidelines are referred to as Biosafety Levels 1 through 4. Each increasing number represents increased risk, requiring more stringent work practice and increasingly protective safety equipment and barriers. A copy of the Guidelines can be obtained from the CDC or accessed online at:http://www.cdc.gov/OD/ohs/biosfty/bmbl5/bmbl5toc.htm

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Biosafety Level 3

Biosafety level 3 practices, safety equipment, and facility design and construction are applicable to microbiology laboratories that work with indigenous or exotic agents with a potential for respiratory transmission, and which may cause serious and potentially lethal infection. Mycobacterium tuberculosis is assigned to this biosafety level. At biosafety level 3, laboratory manipulations should be performed in a Class l or Class ll biosafety cabinet (BSC) or other physical containment device. Secondary barriers include controlled access to the laboratory and ventilation requirements that minimize the release of infectious aerosols from the laboratory. Secondary barriers should include self-closing double-door access and negative airflow into the laboratory. Exhausted air must not be recirculated.

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