|Case Study Three - Selected Cells|
List panel cells to test for ruling-in or ruling-out remaining antibodies in Case Study Three. These would be your selected cells. For rule-out, selected cells should be negative for the antigens that correspond to the antibodies you have possibly identified. In this case, the selected cells for rule-out should be antigen-negative for K and Fya. If you are trying to rule in a possible antibody like K, then the panel cell should be positive for that corresponding antigen so that reactions will occur if the antibody is present.Panel cells 1 and 7 could be used for rule-in of K.Panel cells 2, 4, 5, 6, and 9 can be used for rule-outsPanel cell 2: to rule out C, e, Fyb, Jka, N, s Panel cell 4: to rule out Jka, Lea, N, SPanel cell 5: to rule out C, e, Jkb,MPanel cell 6: to rule out E, Jkb, Lea, N, and sPanel cell 9: to rule out M, S
|Case Study Four- Selected Cell Panel 2|
No reactions were seen with panel cell 1 and 2. This rules out M,N,and s.Notice that C, E, Fya, and P1 are not present on these cells so as not to interfere with ruling-in or ruling-out of the remaining antibodies.ConclusionThrough the processes of ruling-in and ruling-out and matching of reaction patterns, the antibodies that are identified are C,E, Fya, and P1.If the patient has not been transfused in the past 3 months, antigen typing for Fya may be done to further confirm the presence of Fya.Elution and autoadsorptions may also be necessary to confirm the presence of C,E, Fya and P1.