Reticulum Information and Courses from MediaLab, Inc.
These are the MediaLab courses that cover Reticulum and links to relevant pages within the course.
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| Which of the following statements characterize a reticulocyte? (Choose ALL of the correct answers) | View Page |
| All of the following statements concerning Dohle bodies are true EXCEPT: | View Page |
| Dohle Bodies Dohle bodies are small blue irregular staining patches of cytoplasm seen near the edge of the cell in granulocytes under certain conditions. Electron microscopic examination reveals that they are composed of aggregates of rough endoplasmic reticulum remaining from an earlier stage of development. | View Page |
| More on May-Hegglin May-Hegglin bodies stain blue and appear to have a more definite shape than Dohle bodies. When examined under electron microscopy, they appear to be aggregates of thread-like structures in a crystal-like arrangement. May-Hegglin inclusions are RNA material believed to be derived from the endoplasmic reticulum.
May-Hegglin bodies can be seen in monocytes and platelets as well as in all mature granulocytes.
The platelets in May-Hegglin anomaly are very bizarre in appearance and thrombocytopenia is usually noted.
When examining a slide that has bizarre platelets and blue-appearing bodies in the cytoplasm, thought should be given to the possibility of the May-Hegglin anomaly. | View Page |
| The globular inclusions in this cell are fat droplets. | View Page |
| Toxic granulation noted in the neutrophils' cytoplasm reflects an increase in activity of the: (more than one answer may be correct) | View Page |
| Chediac-Higashi anomaly In 1952 Chediak (a Cuban physician) reported a childhood disorder in which abnormal cytoplasmic inclusions appeared in the neutrophils of four family members. In 1954 Higashi reported a similar abnormality in an 11-month old Japanese infant. These inclusions were identified as lysosomal in origin and found in this rare autosomal recessive disorder Death was usually related to recurrent infections or hemmorhage though now some of the affected patients live to reproduce. Ocular and cutaneous albinism, increased susceptibility to pyogenic infections, abnormal granules in neutrophils, and a bleeding tendency are prominent findings. The striking neutrophilic inclusions appear as coarse intra-cytoplasmic azurophilic granules (see photograph).These granules arise from dilated portions of the Golgi-endoplasmic reticulum lysosomal apparatus. Aleutian mink and other animals are known to have Chediak-Higashi syndrome. Azurine pelts from infected mink were once prized by coat makers. | View Page |
| The May -Hegglin anomaly Illustrated in the upper photograph is a cytoplasmic inclusion somewhat resembling a Doehle body. Note that this inclusion is well defined and there is no evidence of toxic granulation in the cytoplasm. When Doehle-like bodies are identified, May-Hegglin anomaly should be considered in the differential diagnosis even though this entity is rare. The May-Hegglin anomaly is an inherited dominant condition in which large 2 - 5 um, basophilic and pyronophilic inclusions are present in granulocytes, including neutrophils, eosinophils, basophils, and monocytes. Similar to Doehle bodies, the May-Hegglin inclusions also are composed of RNA, probably derived from the rough endoplasmic reticulum. May-Hegglin anomaly includes giant platelets containing few fine granules (lower photograph). Sometimes the platelets have bizarre shapes and variable sizes. Variable degrees of thrombocytopenia complicated by mild bleeding problems and purpura may accompany the aberrant platelets. | View Page |
| Case History A 17-year-old young woman was admitted to the hospital with abdominal pain and a tentative diagnosis of appendicitis.The total white blood count was 14,500 cells/cumm with a left shift and neutrophils with changes tagged by the arrow in the photographs (see blue arrow).The bluish-staining, blurred accumulations in the cytoplasm (Doehle bodies), are located at the cell periphery in neutrophils with toxic changes.Doehle bodies are remnants of endocytoplasmic reticulum and are products of cytokine activity in the induction and shortened activity of neutrophil activation.They are often present in conditions with increased neutrophil lysosomal activity, manifest as toxic granulation.In this case, the presence of Doehle bodies serves as markers for infection-induced leukocytosis and supports the diagnosis of acute appendicitis. | View Page |
| Doehle Bodies: Review Doehle bodies are discrete, round or oval aggregates at the cytoplasmic periphery of neutrophils (blue arrows in figures). They stain sky blue with Romanowsky's stain and often may be deceivingly inconspicuous. In electron-micrographs, Doehle bodies are recognized as lamellar aggregates of rough endoplasmic reticulum. Although not considered a marker for leukemia, Goudsmit, et al (Brit J Hematol 20:447-562, 1971)reported their presence in family members, 2 sisters and 3 brothers. Two of the brothers died of acute myeloblastic leukemia. These testimonials indicate that Doehle bodies, when identified in peripheral blood smears, should be taken seriously so as to stimulate a clinical investigation of the patient. | View Page |
| The cell photographed here is known as a MOTT cell. The condition in which these cells are associated is: | View Page |