Reannealing Information and Courses from MediaLab, Inc.
These are the MediaLab courses that cover Reannealing and links to relevant pages within the course.
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| Factors Affecting Hybridization Because hybridization involves nucleotide bases and the separation and joining or reannealing of strands, several environmental factors can influence this process: Temperature: If the temperature is too high, the strands melt. If it is too low, they might be forced together. The pH: A pH that is too alkaline will cause the strands to separate; too acidic and they are forced together. The guanine to cytosine ratio (G:C ratio): Since this bond is stronger than the other nucleotide bonds, if the G:C ratio in the desired target strand is high, the separation process may take longer. | View Page |
| Thermal Cycling Thermal cycling (thermocycling) uses specific instrumentation to cause variations in temperature during an amplification procedure in order to produce copies of the target of interest (or amplify the target of interest) so that detection can take place.The temperature variations increase to unfold the DNA and then decrease to allow for reconnection or reannealing. | View Page |