Reading Gram Stained Smears From Cultures (Online Course)
Betty Smith, MT(ASCP); Mary Ann Fiene, MSMT(ASCP); Rukhsana Evans, MSMT(ASCP)
This course explains how to intelligently evaluate and interpret gram stained smears from cultures. Good for clinical laboratory science students, cross training, and CE. Covers gram positive and gram negative bacilli; single, pair, chain, and tetrad cell arrangements; and reporting.
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Continuing Education Credits
- P.A.C.E.® Contact Hours: 1.5 hour(s)
- Florida Board of Clinical Laboratory Science CE - General (Microbiology/Mycology/Parasitology): 1 hour(s)
Objectives
- Explain the importance of Gram stained smears from isolated colonies.
- Discuss the procedure for reading smears from cultures with respect to:
- Gram stain reaction
- Shape of the organism
- Arrangement of cells
- Technical errors
- Identify microorganisms as gram positive or gram negative and be able to describe their shape and cell arrangement.
- Be able to read smears from various cultures, and write an appropriate report for each.
Course Outline
Click on a link in the outline to view a sample page from this course.
- Introduction to Reading Gram Stained Culture Smears
- General Aspects of Gram Stained Culture Smears
- Gram Stain
- Cellular Arrangement
- Gram Positive and Negative Stain Colors
- Focus Power
- Control Slides
- Macroscopic Appearance
- Cocci and Rods
- Variations in the Shape of Cocci
- Gram Negative Bacilli
- Bacilli Size and Shape
- Variations in the Ends of Rods
- Budding Forms of Yeast
- Single Cell Arrangement
- Pair Cell Arrangement
- Chain Cell Arrangement
- Tetrad Cell Arrangement
- Clusters of Gram Positive Bacteria
- Yeast Cells
- Summary
- Please select all the choices below than correctly describe this Gram stained smear.
- Please select all the choices below than correctly describe this Gram stained smear.
- Please select all the choices below than correctly describe this Gram stained smear.
- Please select all the choices below than correctly describe this Gram stained smear.
- Please select all the choices below than correctly describe this Gram stained smear.
- Please select all the choices below than correctly describe this Gram stained smear.
- Please select all the choices below than correctly describe this Gram stained smear.
- Which of the following terms can be used to describe cocci?
- Bacilli can have tapered or rounded ends.
- Reporting Gram Stained Culture Smears
- Principle
- Information to be reported
- Please select the correct choice for reporting.
- Please select the correct choice for reporting.
- Please select the correct choices for reporting.
- Please select the correct choice for reporting.
- Please select the correct choice for reporting.
- Please select the correct choice for reporting.
- Please select the correct choice for reporting.
- Please select the correct choice for reporting.
- All of the following information is included in a culture smear report EXCEPT:
- The type of culture medium has no effect on cellular arrangement.
- The bacteria on the positive and negative control slides stain pink. This does not affect the rest
- Observations that can be of value to the microbiologist include:
Additional Information
Level of Instruction: Basic
Intended Audience: Clinical laboratory science students, and medical tehcnologists and technicians seeking review, cross training, or continuing education opportunities. It is also appropriate for medical students and pathology residents.
Course Description: This illustrated course cover the basics of reading gram stained smears from cultures, including interpretation of controls, reading, interpreting and reported gram stained culture smears. It is illustrated with numerous photomicrographs of actual culture gram stains, and contains numerous interactive quiz questions.
About the Course: This course is part of a series of courses adapted for the web by MediaLab Inc. under license from Educational Materials for Health Professionals Inc. Dayton OH, 45420. Copyright EMHP.
Reading Gram Stained Smears From Cultures Keywords
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