Pulsed Information and Courses from MediaLab, Inc.
These are the MediaLab courses that cover Pulsed and links to relevant pages within the course.
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| Types of Electrophoresis There are numerous applications of electrophoresis. Routine protein electrophoresis performed in clinical laboratories is the oldest method and therefore the most frequently used method. With the advent of molecular diagnostics, several other electrophoresis methods have become very important, highly automated, and have several important applications.Types of electrophoresis that will be discussed are Routine electrophoresis High resolution electrophoresis Polyacrylamide gel electrophoresis Capillary electrophoresis Isoelectric focusing Immunochemical electrophoresis Two-dimensional electrophoresis Pulsed field electrophoresis | View Page |
| Pulsed Field Electrophoresis Larger fragments of DNA, > 50 kilobases (kb), cannot be separated with AGE or PAGE in routine electrophoresis systems; the gel pore sizes are too small for their migration. Fragment separation can be achieved with alternately applying the power to different pairs of electrodes. The most common method alternates the positive and negative electrodes in cycles during electrophoresis. The DNA fragments must reorient to a new field direction in each cycle. These changing pulses and reorientations separate the large size DNA fragments.Sample runs require longer time periods, some 24 hours or more, special gel boxes, different electrodes and controls for switching the electric fields during electrophoresis. | View Page |
| Currently there has been a revitalization in the clinical usage of electrophoresis. Previously, methods were primarily used to separate proteins in blood and other body fluids. From the following statements, select the statements that correctly describe newer applications of electrophoresis. | View Page |
| Electrophoresis and Molecular Diagnostics Because of ionized phosphate groups, both DNA and RNA will migrate in an electrical field with an appropriate buffer. They are negatively charged and will migrate to the anode. The speed of migration and separation achieved is based upon size with smaller molecules traveling faster. The shape of macromolecules, type of support medium, and electrophoresis method also vary the separation results. The isolated nucleic acid can be single-stranded or double-stranded and can fold into other structures. AGE, PAGE, and CE are the most common electrophoresis methods used in analysis of nucleic acids. Pulsed electric fields are needed to separate large fragments. The electrophoresis employed in blotting techniques enhance these discrimination techniques. | View Page |