Probes Information and Courses from MediaLab, Inc.
These are the MediaLab courses that cover Probes and links to relevant pages within the course.
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| Human Genome Much research has been conducted to identify the alphabet of the human cellular language otherwise known as the human genome. This identification or roadmap of the human genetic material has opened the door to the mainstreaming of molecular diagnostics within the clinical laboratory setting.While the mapping of the human genome project is complete, many times it is not necessary to be able to identify the entire sequence; rather, we can use the specific portion of the code that is unique to the disease or condition in question. These short portions of the genetic molecular sequence or oligonucleotides, can then be used as probes to seek out and detect or amplify the target sequence. | View Page |
| Hybridization Hybridization is the pairing or annealing of two strands of DNA. Hybridization is therefore based on the formation of double stranded hybrids from single stranded nucleic acids. These double stranded hybrids form under precise conditions and are detected using probes. A probe is a set of nucleic acids of known identity which seeks out the target of interest. Depending on the detection technique, probes and/or targets can either be labeled or unlabeled and the reaction can take place with one attached to a matrix or in solution, thus dividing the techniques into two broad categories: Solid phase Solution phase | View Page |
| Detection Detection techniques can vary in both direct and amplified methodologies and can include labeling either the probe or the target molecule of interest:Chemiluminescence: Release of light energy at the end of a chemical reaction that is detected by a luminometer. Uses a label such as acridinium ester. Electrophoresis: movement in a matrix such as a gel that is caused by an electrical field.Enzyme: Uses enzyme and substrate principles to label the appropriate target or probe. Can be combined with fluorescence or dyes for detection.Fluorescence: Molecules that emit light at a longer wavelength when excited at a shorter wavelength. Detection techniques include fluorescent staining of nucleic acids as well as fluorescent labeled probes that are measured in a fluorometer or with fluorescent polarization.Radioactivity: Uses a labeling technique where the radioactive label is then measured in a scintillation counter. The earliest assays utilized radioactive decay. | View Page |
| Which statement(s) are true about Root Cause Analysis? | View Page |
| RCA Considerations RCA considers the following elements:
Factors associated with an adverse eventStudy of specific processes related to the eventPotential improvement processes to reduce the likelihood repeating the eventQuestioning that probes deeply by repeatedly asking "Why?"Analysis that is thorough and credible
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| Procedures for the rapid culture confirmation of suspected colonies of B. dermatitidis, C. immitis and H. capsulatum recovered from clinical specimens include: | View Page |