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Polychromasia Information and Courses from MediaLab, Inc.

These are the MediaLab courses that cover Polychromasia and links to relevant pages within the course.

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CLIA Hematology / Hemostasis Review
An abnormal variation in the size of the red blood cells is termed:View Page
Which of the following observations would best explain why a peripheral blood smear is exhibiting polychromasia:View Page

Erythrocyte Inclusions - Wright Stained Smears
This smear shows:View Page
This smear shows:View Page
What abnormality is present in this slide?View Page
What abnormality is present in this slide?View Page
What abnormalities can be seen in this slide?View Page
What abnormalities are present in this slide?View Page
What abnormality is present in this slide?View Page
The red cell in the center of the field contains a:View Page
Reticulocytes

Although the nucleus has been extruded, the reticulocyte is still considered immature because it retains numerous organelles needed for hemoglobin production, such as ribosomes, mitochondria, and fragments of the Golgi apparatus. The reticulocyte is slightly larger (10 microns) than the mature erythrocyte. A reticulocyte normally remains in the bone marrow for one or two days before entering the circulation and its final 24 hours of maturation. The red cell is mature when hemoglobin production is complete and the organelles have disintegrated. Reticulocytes normally make up 0.5 - 1.5% of the peripheral blood red cells. They appear blue/gray on the Wright's stained smear. The residual RNA in the cytoplasm causes the blue/gray color. The terms, polychromasia or polychromatophilic, are used to describe these cells on a Wright's stained preparation. A supravital stain such as new methylene blue N or brilliant cresyl blue is used to stain reticulocytes for an actual count.

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The cells in this slide show:View Page
The abnormalities that are seen on this slide are probably associated with which of the following conditions?View Page
The cells in this slide show polychromasia.View Page
What is Erythrocyte inclusion?

Erythrocyte inclusion is a term used to describe structures that may be present in red cells. The inclusions discussed in this course are: reticulocyte - Wright-Giemsa stain (polychromasia)basophilic stipplingHowell-Jolly bodiesPappenheimer bodies (Wright-Giemsa stain)siderotic granules (iron stain)Cabot rings

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Red Cell Disorders: Peripheral Blood Clues to Nonneoplastic Conditions
The condition most likely associated with the peripheral blood picture shown in the photograph is:View Page
The blood study from which this smear was obtained revealed an MCV of 115 femtoliters (fl).Normal MCV values in adults= 80 - 90 fl.Normal MCV values in full-term infants= 98 -108 fl.Which of the following conditions may be indicated by the results seen on this peripheral blood smear?View Page
Guidelines for standard reports

In a study on the reporting of red blood cell morphology abnormalities conducted in Ontario, Canada (Hookey L, Dexter D, Lee DH, Laboratory Hematology 7:83-88, 2001), fewer than 50% of 33 participants used the same term to describe the quantitative frequency of peripheral blood abnormalities. Seven blood smears, each containing one of several abnormal erythrocytes-- schistocytes, teardrop cells, acanthocytes, and Howell-Jolly bodies--were evaluated by 32 participants. The participants were asked to document their evaluations from a list of quantitative terms. There was a heterogeneity in the use of terms "rare," "slight," "occasional," "few," "mild", "present," "moderate," "many," and "marked." Choices of terms were subjective without points of reference. Guidelines for establishing standardized qualitative estimations of abnormal erythrocytes in the peripheral smear are presented as follows: 1+ = 2 - 4/Oil Immersion Field (OIF) 2+ = 5 - 7/OIF 3+ = 8 - 10/OIF 4+ = >10/OIF. The terms "few," "moderate," "many," and "marked" may be substituted for the 1+ - 4+ grading system, but only when their specific points of reference are universally understood in tandem with the above guidelines. A comment should be triggered if any erythrocyte abnormalities are seen in numbers >3/OIF including, but not limited to, polychromasia, basophilic stippling, nucleated RBC's, and Howell-Jolly bodies. Rouleaux or RBC agglutination are important findings and must be documented.

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Red Cell Morphology
Which of the following forms is seen in this field?View Page
Color of Erythrocytes

In addition to the amount of hemoglobin present, the color of the cell must also be considered. Completely mature red cells appear buff-colored, while slightly immature non-nucleated red cells (reticulocyte stage) appear blue/gray on Wright's stained smears due to the presence of residual ribonucleic acid (RNA).The terms used to describe these cells are polychromasia or polychromatophilia. Polychromatophilic cells are frequently larger in size than mature red cells and can be distinguished from both types of macrocytes by this distinctive color.

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Polychromasia

The large cell in the center of this field is slightly blue/gray and is an example of a polychromatophilic red cell.Increased numbers of these cells, (approximately 2 or greater per oil immersion field,) indicate increased red cell output by the bone marrow. Polychromatophilic cells are larger and younger than mature red cells, and may be larger than 9 micron in diameter. Under normal conditions, these young red cells remain in the bone marrow one or two days before release into the bloodstream. However, when the bone marrow is stressed due to blood loss or other conditions, these cells are prematurely released into the blood, resulting in a blood smear with polychromasia. These red cells are often referred to as shift cells. If stained with a supravital stain, they would be identified as reticulocytes.

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