Poking Information and Courses from MediaLab, Inc.
These are the MediaLab courses that cover Poking and links to relevant pages within the course.
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| Support Media Care is required in handling electrophoresis support media. Touching the gel can leave harmful fingerprints or poking the gel in sample application can damage the formation of separated bands. Some gels require presoaking; these need to be lightly blotted because the excess water can mar band formation. | View Page |
| Troubleshooting Irregular, Distorted or Atypical Bands Band Appearance Possible Cause Short Migration Patterns Contaminated or Aged Buffer Diffuse Bands Markedly Wet Gels Poor Sample Application Streaks Perpendicular to Bands Tearing/Poking Gel in Sample Application Weak Bands Not Enough Sample Not Enough Stain Other Causes of Irregular and Distorted Bands Hemolyzed Sample Bent or Dirty Applicator Air Bubbles in Sample Application Too Much Sample Wick Flow Too Much Heat or Drying | View Page |