Picking Information and Courses from MediaLab, Inc.
These are the MediaLab courses that cover Picking and links to relevant pages within the course.
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|Example- Choosing Selected Cells|
The selected cells should be antigen-negative for the antibody that you think is present and antigen-positive (homozygous) for what you are trying to rule out. You are designing a panel that addresses your testing needs. Example: JkbIf you suspect that your patient has an anti-Jkb and further rule out cells are needed, then those rule out cells should be negative for Jkb. In the table below, donor cells 1,2, 4, 6, 9 and 10 may be used when creating a select panel to test the patient and help rule out the remaining possible antibodies. The homozygous rule applies when choosing which cells to use for testing (antigens highlighted in light-yellow).Example: Picking cells to rule out CUse panel cell 1 and panel cell 2 (C is in the homozygous state). Explanation: Panel cells 1 and 2 do not contain the antigen Jkb (signified by "0" on cell panel). If these cells are tested with the patient's plasma and the reaction is negative, it can be assumed that the patient does not have an antibody to C. C is now ruled out because there would be a total of 3 negative patient reactions with C positive cells (These two reactions and screen cell I from the antibody screen, shown again below). This should be done for all clinically significant antibodies that you were unable to rule out on the first panel.
|Picking Selected Panel Cells Conservatively|
Choose cells that can help rule out more than one antibody at a time in order to help decrease supply usage and tech prep time. Example: Ruling out C, Fyb, and M if you have a suspected JkacCFyaFybJkaJkbMNPanel cell 90+0+0++0Panel cell 100+++0+0+Panel cell 11++0+0++0Panel cell 12++++0++0Instead of running 3 separate cells to rule out the antibodies, you can choose one that is homozygous positive for M, C, Fyb and negative for Jka. Panel cell 9 works in this case.If the only antibody that is present is Jka, then your test results should be negative. If the results are positive then further rule outs will be needed to determine what is present.
|Observed collection scenarios|
Scenario 1:A donor was asked to wash her hands prior to picking out the drug screen collection kit. The collector noticed that the donor was washing only one hand.Collector's response:The collector tells the donor that not washing both hands is an indication of possible interference with the testing process and that it could be interpreted as a refusal to test. If the donor still refuses to wash both hands, the collector must stop the collection process, note the refusal on the CCF and notify the DER.Scenario 2:The donor was asked to remove his hat before going into the restroom. As he reluctantly did so, it was noticed that he was trying to conceal a container that was hidden inside the hat.Collector's response:The collector first explains the circumstances to a supervisor. If the supervisor concurs that an observed collection should be done, the collector then tells the donor that a directly observed collection will be conducted because his conduct indicated a possible attempt to adulterate, substitute, or dilute the specimen. The collector marks on the CCF that the collection was observed and notes under Remarks why it was observed.
|Category A Packages - Shipper's Declaration For Dangerous Goods|
A "shipper's declaration for dangerous goods" form must be filled out for category A packages, but is NOT required for category B or exempt packages. The shipper's declaration must be handed to the courier picking up the package; do not place it inside the box. The number of copies that are required varies according to shipper, but you should always keep one copy. The shipper must retain a copy of the Shipper's Declaration Form for at least two years. Do not use white out or erasures to make corrections on a Shipper's Declaration Form. If a correction must be made, cross out the incorrect information, using a single line, and sign the correction.Fed Ex requires that the Dangerous Goods forms be filled out with software using dangerous goods compliance editing checks. This will be discussed further on the following page.If dry ice is included in a package containing a category A substance, it should also be listed on the shipper's declaration for dangerous goods. However, it is NOT necessary to fill out this form if dry ice is included in category B or exempt packaging; details about the dry ice are included instead on the air waybill.
A category A infectious substance is in a form that is capable of causing permanent disability or life-threatening or fatal disease in otherwise healthy humans or animals when exposure to it occurs. Exposure would occur if the substance were released from its protective packaging and a human or animal came into contact with it. Therefore, it is critical that a category A infectious substance does not end up in the hands of an unauthorized individual who may purposely or unknowingly release the substance from its protective packaging and endanger humans or animals. Being aware of the people that you interact with in the process of packaging and sending category A substances is vital to the safety of the transport and prevention of a health disaster. An outsider with limited access and system knowledge could constitute a threat, but be aware that insiders could also be a threat, e.g., a disgruntled employee or a person who is angry with his or her supervisor or job or the government. Anyone desiring to do harm could potentially seize the opportunity to steal a hazardous material.Follow these precautionary procedures: When you are questioned about an infectious substance that you are packaging for shipment, it is important that you know the person that is asking AND that he or she has a need to know. If you do not know the person and if you are not aware that the person needs to know about the substance that is being shipped, do not answer the questions. You could refer him or her to your supervisor. Watch for unusual behavior. Secure the package until it is picked up. Check the identification of the courier who will be picking up the package. Use an intralaboratory chain of custody procedure if the specimens are tranferred within the facility or system. Track the package once it has been sent to be sure it arrives safely. Notify the Responsible Official or federal authority if the package does not arrive at its destination.