Peroxide Information and Courses from MediaLab, Inc.
These are the MediaLab courses that cover Peroxide and links to relevant pages within the course.
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| Glucose Test The test for glucose is a double sequential enzyme reaction, utilizing the glucose-oxidase/peroxidase method. In the first reaction, glucose oxidase catalyzes the oxidation of glucose to gluconic acid and hydrogen peroxide. Then, the peroxidase catalyzes the oxidation of a chromogen by the hydrogen peroxide to form a colored product. This method does not react with lactose, fructose or galactose. Study the dipstick color chart to become familiar with the range of color changes. The urine specimen should be at room temperature for these enzyme reactions to occur properly. | View Page |
| False Positive Results False positive results can be attributed to strong oxidizing agents such as hydrogen peroxide (H2O2) or bleach (hypochlorite). | View Page |
| Which of the following substances can result in a false negative urine glucose reagent strip test result? (Choose ALL of the correct answers) | View Page |
| False Positive Results A false positive result for blood on the reagent strip can occur when oxidizing contaminants, such as hypochlorite (bleach), remain in collection bottles after cleaning. Contamination of the urine with provodine-iodine, a strong oxidizing agent, used in surgical procedures can result in a false positive reaction. Microbial peroxide found in association with urinary tract infections may also cause false-positive results. Capoten® (Captopril) can cause decreased reactivity. The muscle tissue form of hemoglobin, myoglobin is a well-known cause of false-positive reactions on the blood portion of the reagent strip. When tissue hemoglobin is present, the urine specimen has a clear red appearance. Patients suffering from muscle-wasting disorders or muscular destruction due to trauma, prolonged coma, or convulsions or individuals engaging in extensive exertion may have myoglobin in their urine. Specific tests for myoglobin, such as immunodiffusion techniques or protein electrophoresis, are needed to confirm the presence of this substance in a urine specimen. Levels of ascorbic acid normally found in urine do not interfere with this test. | View Page |
| The Superoxol Test Superoxol is an additional spot test that may be helpful in the presumptive identification of N. gonorrhoeae.Superoxol is 30% hydrogen peroxide, in contrast to the 3% solution that is used in the catalase test.Other Neisseria species and Moraxella catarrhalis are either negative for this test or give a weak, delayed reaction. | View Page |