Oxidation Information and Courses from MediaLab, Inc.
These are the MediaLab courses that cover Oxidation and links to relevant pages within the course.
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| Glucose Test The test for glucose is a double sequential enzyme reaction, utilizing the glucose-oxidase/peroxidase method. In the first reaction, glucose oxidase catalyzes the oxidation of glucose to gluconic acid and hydrogen peroxide. Then, the peroxidase catalyzes the oxidation of a chromogen by the hydrogen peroxide to form a colored product. This method does not react with lactose, fructose or galactose. Study the dipstick color chart to become familiar with the range of color changes. The urine specimen should be at room temperature for these enzyme reactions to occur properly. | View Page |
| False Negative Results False negative results occur when elements present in the urine interfere with either the enzymatic reaction or prevent the oxidation of potassium iodide. Examples of such substances include: large quantities of ketones aspirin ascorbic acid > 50 mg/dL with some reagent strips levadopa 5-hydroxyindoleacetic acid homogentisic acid sodium fluoride ( a preservative)A specific gravity higher than 1.020 may lower glucose reagent sensitivity, especially in the presence of a high urine pH. Exposing reagent strips to excess humidity may also reduce glucose reagent reactivity.Check the package insert of the reagent strips used in your laboratory for interfering substances that may affect glucose results. | View Page |
| A urine specimen to be tested for bilirubin arrives in the laboratory after sitting on the counter at the nurses station for 2 hours. Which of the following statements describes the Ictotest® reaction that could potentially occur in this situation? | View Page |
| The principle of the Ictotest® for bilirubin is based upon: | View Page |
| Drug Metabolism The liver plays a major role in converting lipophilic nonpolar molecules (drug molecules) to more polar, water-soluble forms through a series of enzymatic reactions. Drug molecules can be modified by either phase I or phase ll reactions. Phase I reactions alter chemical structure by oxidation, reduction, or hydrolysis. Phase ll reactions conjugate drugs to create products that are water-soluble. | View Page |
| Abnormal forms There are a number of abnormalities of sperm morphology.
Abnormal heads can include enlarged head, double head, round head, constricted head, amorphous head, pinhead, and acute tapering forms. There are also heads with abnormal numbers of vacuoles.
Midpiece abnormalities include distended and thin midpiece regions.
Abnormal tails include short tails, double, triple or multiple tails, coiled tails, broken tails, or absent tail.
Cytoplasmic droplets are also seen in some specimens. These are large regions of cytoplasm just below the head assumed to represent failure of complete sperm maturation or a sign of either toxicity or oxidation. There have also been reports that cytoplasmic droplets may be artifacts from the fixation and staining for morphology analysis.
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