Nitroprusside Information and Courses from MediaLab, Inc.
These are the MediaLab courses that cover Nitroprusside and links to relevant pages within the course.
Learn more about laboratory continuing education for medical technologists to earn CE credit for AMT, ASCP, NCA, and state license renewal and recertification. Or get information about laboratory safety and compliance courses that deliver cost-effective OSHA safety training and continuing education to your laboratory's employees.
| Testing for Ketone Bodies Testing for ketone bodies is based on a nitroprusside reaction. Acetoacetic acid reacts with sodium nitroferricyanide and glycine in an alkaline medium to produce a violet-to-purple colored complex. The reagent strip method can detect as little as 5 mg/dL acetoacetic acid in urine. It does not react with acetone unless glycine is present or B-hydroxybutyric acid. Since these two compounds are derived from acetoacetic acid, their presence can be assumed if the test for ketones is positive. Ketones are reported either as negative, small, moderate or large amounts; or negative, 1+, 2+, 3+, or 4+. In some severe cases of ketosis, it may be necessary to perform tests on serial dilutions to provide more information on the quantity of ketones present. | View Page |
| The ketone component that is measured by the nitroprusside reaction is: | View Page |
| The reagents used in the nitroprusside reaction are: (Choose ALL of the correct answers) | View Page |
| Cystine Crystals Cystine crystals indicate an abnormality in metabolism of the amino acid cystine. These crystals appear as colorless, refractile, hexagonal plates with even sides. Cystine crystals sometimes occur in pairs. They produce a red color in the nitroprusside reaction. Cystine may be confused with the hexagonal forms of uric acid but does not polarize light. Cystine crystals occur in acid urine. | View Page |