Mycology: Yeasts and Dimorphic Pathogens (Online Course)

Elmer W. Koneman, MD

Mycology: Yeasts and Dimorphic Pathogens uses an interrogative, question-and-answer, approach to direct and instruct the participant in identifying yeast and dimorphic pathogens. Numerous images enhance the descriptions of identifying characteristics by illustrating the appearance of fungal colonies on media and microscopic appearance. The course also identifies sources of infection and associated diseases.

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Continuing Education Credits

• P.A.C.E.® Contact Hours: 1.5 hour(s)
• Florida Board of Clinical Laboratory Science CE - General (Microbiology/Mycology/Parasitology): 1.5 hour(s)

Objectives

• Identify yeast species by observing the appearance of the colony morphology and through microscopic appearance.
• Discuss various sources of fungal infections and the diseases that are associated with yeasts and dimorphic molds.
• Identify dimorphic fungal species by observing colony growth on plates and slants and through microscopic appearance.
• Utilize appropriate laboratory methods to aid in the identification of fungal species.
• Correlate dimorphic fungi with their corresponding yeast forms.

Course Outline

Click on a link in the outline to view a sample page from this course.

• Yeasts
• Match the names of each of the yeast species with its most likely colony morphology as seen in the images on the right.
• Match the name of each of the species of yeast listed with its corresponding microscopic appearance as illustrated in the images on the right.
• Match the names of each of the yeast species listed with its corresponding appearance when grown in cornmeal agar, as seen in the images.
• Match the names of each of the species of yeast listed with its associated phenotypic property that is helpful in establishing a species identificatio...
• Match each of the microscopic structures listed in the drop-down box with the name of its corresponding yeast species on the right.
• Match the name of each species of yeast listed below with the location listed in the drop-down box where that species may be concentrated.
• Arrange the yeast species listed in the drop-down box in order of increasing virulence, from the least to the most pathogenic.
• Arrange in sequence the steps that should be taken to make a definitive identification of Cryptococcus neoformans.
• Match the complications that are most likely to be associated with each of the two yeast diseases that are listed in the drop-down box:
• Match each of the fungal species listed below with the appropriate category, indicating whether or not it has the capability of producing pseudohyphae...
• The colonies shown in this photograph were grown on Guizotia abyssinica (bird seed) agar at 30°C for 72 hours. The most likely identification is:
• The growth of the yeast-like colonies shown in the upper image was obtained on blood agar from a skin culture only in the area overlaid by virgin oliv...
• The forms seen in this photomicrograph, produced from a light inoculum of an unknown yeast colony incubated in rabbit plasma at 35°C for 2 hours, lead...
• Shown in this photomicrograph is a Gomori methenamine silver stain of a lung biopsy obtained from a patient with X-ray evidence of multi-focal pneumon...
• The colony shown in the upper image was recovered from peritoneal fluid of a patient receiving continuous peritoneal dialysis. The lower image is a ph...
• The colonies illustrated in this photograph were recovered from a blood culture after 48 hour incubation at 30°C. The most likely source for the septi...
• Although only a few human cases have been reported, brewers and bakers may in particular be at increased risk for developing infections with:
• Of the following responses, the one observation that would rule out cryptococcosis as the cause of meningoencephalitis is:
• The most common eye complication in patients with candidiasis is:
• A hematology technologist observed the intracellular forms seen in the field of view of a Wright-Giemsa-stained peripheral blood smear shown in this p...
• This photomicrograph is an acid-fast stained smear prepared from a yeast colony growing on ascospore agar. A helmet-shaped, red-staining, acid fast ye...
• Illustrated in this photomicrograph of a lactophenol blue preparation of a urine sediment is a cluster of yeast cells that were presumptively identifi...
• This photomicrograph is a representative field of a Wright-Giemsa-stained bone marrow aspirate in which a pair of budding yeast cells is seen centrall...
• Oral candidiasis may be directly exasperated by the habitual ingestion of:
• The ability of Candida albicans to alternate between two phenotypes, which may be related to the virulence of this species, is called:
• A yeast identification system gave a biotype number for an unknown isolate that did not differentiate between Candida tropicalis and Candida parapsilo...
• Each of the following is considered to be a virulence factor in Cryptococcus neoformans except:
• Dimorphic Pathogens
• Match each of the names of the dimorphic fungal species with its corresponding mold form as seen in the photomicrographs.
• Match the names of the species of dimorphic fungi listed in the drop-down box with its corresponding yeast form as illustrated in the images.
• Match each of the names of the dimorphic fungi listed with the names of the animals that most commonly may be related to transmission of disease to hu...
• Match the name of each of the dimorphic fungi listed with the corresponding activity by which infection can be avoided.
• Match each of the diseases listed in the drop-down box with the name of its most likely associated dimorphic fungal species.
• Arrange the fungal species that are listed in the drop-down box according to length of time of recovery in primary culture; from most rapid to the slo...
• Match the names of each of the diseases listed with its appropriate situation:
• Match the names of each of the diseases listed with its appropriate geographic area:
• The colonies shown in the upper image were obtained on blood agar from a sputum specimen after 10 days incubation at 30°C. The lower image is a photom...
• The growth of the colonies shown in the upper image was obtained on blood agar from a sputum specimen after 8 days of incubation at 30°C. The lower im...
• The colonies shown in the upper image, obtained from a biopsy of an ulcerating skin lesion of the arm, are growing on agar slants of Sabouraud's dextr...
• One of the characteristics common to the dimorphic molds is the ability to convert the mold forms to the yeast forms by incubating subcultures in enri...
• This image illustrates a lactophenol blue mount of a mold recovered after 7 days incubation of brain heart infusion broth. The large macroconidia sugg...
• This image illustrates a lactophenol blue mount prepared from a mold recovered after 7 days incubation on brain heart infusion broth. The individual m...
• Each of the following dimorphic fungal infections have been observed in animals living in their natural environment except:
• Which of the following fungal infections was once known as "Chicago disease" because so many cases had occurred in the Chicago area?
• Procedures for the rapid culture confirmation of suspected colonies of B. dermatitidis, C. immitis and H. capsulatum recovered from clinical specimens...
• The colonies growing on the surface of this brain-heart infusion with blood agar plate were "converted" from a mold colony suspected of being Histopla...
• Although care should be taken when working with all fungus cultures in the laboratory, personnel are particularly prone to develop laboratory acquired...
• The ingredient added to culture media to enhance the recovery of the dimorphic fungi by preventing the overgrowth of more rapidly growing, saprophytic...
•   The dimorphic fungus that may produce black, yeast-like colonies after prolonged incubation at 37°C is:

Additional Information