Mycology: Hyaline and Dematiaceous Fungi (Online Course)

Elmer W. Koneman, MD

Mycology: Hyaline and Dematiaceous Fungi uses an interrogative, question-and-answer, approach to direct and instruct the participant in identifying hyaline and dematiaceous fungi. Numerous images enhance the descriptions of identifying characteristics by illustrating the appearance of fungal colonies on media and microscopic appearance. The course also identifies sources of infection and associated diseases. This course is appropriate for laboratory professionals and students in clinical laboratory science programs who want to increase their understanding of mycology including microscopic and colony appearance and isolate identification.

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Continuing Education Credits

• P.A.C.E.® Contact Hours: 1.5 hour(s)
• Florida Board of Clinical Laboratory Science CE - General (Microbiology/Mycology/Parasitology): 1.5 hour(s)

Objectives

• Identify hyaline mold isolates by observing the appearance of the colony on agar plates and through microscopic appearance.
• Discuss various sources of fungal infections and the diseases that are associated with hyaline and dematiaceous molds.
• Distinguish between saprophytic hyaline molds and dimorphic fungi by performing specific laboratory procedures.
• Define these terms that apply to fungi and are used to identify specific species: sporulation, conidia, conidiophore, hyphae, phialide.
• Identify dematiaceous molds by observing characteristic structures and their rate of growth.

Course Outline

Click on a link in the outline to view a sample page from this course.

• Hyaline Molds
• Match each hyaline mold from the drop-down list to its corresponding colony and microscopic description. The mold colonies are illustrated in the imag...
• Match each hyaline mold from the drop-down list to its corresponding microscopic and colony description. The microscopic appearance of the molds are i...
• Match each hyaline mold from the drop-down list to its corresponding colony and microscopic description. The mold colonies are illustrated in the imag...
• Match each of the names of the fungi listed in the left column with its most likely associated disease listed in the right column.
• Match each of the names of the hyaline molds listed with the environmental conditions or natural objects with which it is most likely associated.
• Match the names of each of the species of hyaline molds listed with the appropriate category.
• Match the names of each of the species of hyaline molds listed with the appropriate category.
• Match the names of each of the species of hyaline molds listed with the appropriate taxonomic category.
• Match the names of each of the fungi listed below into the appropriate category indicating the classification of infection with which it is most commo...
• The microscopic features shown in this photomicrograph is of a fungus commonly associated with:
• Based on the structures observed in this photomicrograph, the most probable species of the fungus recovered from an induced sputum specimen is:
• Illustrated in this photograph is a "green lawn" colony of Gliocladium species. The other hyaline mold that produces this type of colony is:
• The fungus illustrated in this photomicrograph was recovered from an induced sputum specimen from a 74 year old man with chronic obstructive pulmonary...
• The dermatophytic fungus shown is in this photomicrograph, recovered from a ringworm lesion of the skin of the back of the hand of an 8 year old boy, ...
• An Aspergillus species was recovered from a sputum specimen of a patient with X-ray evidence of fungal pneumonia. Microscopic examination did not perm...
• The hyaline mold illustrated in this photomicrograph can be identified as:
• The differentiation between Aspergillus species and Scedosporium species may be difficult when only hyphal elements are observed in stained tissue sec...
• A dermatophyte that produces thin-walled, two or three-celled macroconidia, and no microconidia, most likely belongs to the genus:
• The spores produced by telomorphic forms of Aspergillus glaucus are:
• The bare fruiting heads shown here, characteristic of Aspergillus niger, demonstrate a spherical vesicle with phialides positioned around the entire c...
• A dull white fungus, turning mouse gray on maturity, was recovered from material aspirated from a bone cyst in the upper femur. Based on the microscop...
• The "birds on a fence" arrangement of uniform-sized, tear-shaped microconidia is characteristic of:
• Illustrated in this photomicrograph are fruiting heads of Trichoderma species. Note the single, long, tapered phialides (red arrows), extending latera...
• The fungus illustrated in this photomicrograph of a lactophenol blue-stained preparation was recovered from skin scrapings of a patient with tinea ped...
• Illustrated here is a single conidium of Microsporum canis. Note that the hilar cell appears fractured (short red arrow), where it was released from t...
• The hyaline saprobic fungus that has microscopic features similar to the mold form of Histoplasma capsulatum is:
• The hyaline saprobic fungus that has microscopic features similar to the mold form of Coccidioides immitis is:
• Several saprophytic, hyaline molds have microscopic characteristics that mimic the mold forms of the dimorphic fungi (Histoplasma capsulatum, Blastomy...
• The most helpful feature in differentiating the Zygomycetes from the other hyaline molds in the clinical mycology laboratory is:
• Dematiaceous Molds
• Match each of the names of the fungal species listed with the corresponding identifying structures illustrated in the photomicrographs:
• Match the names of each of the fungal species listed in the drop-down box with the corresponding identifying structures illustrated in the photomicrog...
• A presumptive identification of the four genera of slower growing pathogenic dematiaceous molds can be made by observing specific types of conidiation...
• Match the name of each dematiaceous fungus listed in the drop-down box with its most likely disease.
• Match the name of each fungal species listed with its most likely corresponding morphologic features.
• Match the name of each fungal species listed in the drop-down box with its most likely corresponding morphologic feature.
• Match the names of each of the fungi listed with its appropriate category.
• Match the names of each of the fungi listed with its appropriate category depending upon whether the multi-celled macroconidia are divided by both lon...
• Match the names of each of the fungi listed with its appropriate category indicating the degree of pathogenicity.
• The fungal species most likely associated with the granulomatous infection seen in this photomicrograph, illustrating segmented, dark brown-staining g...
• The infrequently encountered mold that is represented by the photomicrograph begins as a gray-white colony that blackens with maturity as the hyphae b...
• The multi-celled conidia of this dematiaceous mold are divided into cells by what are called distosepta (pseudosepta), indicating that the individual ...
• The oval or curved multi-celled, dark-staining macroconidia divided by transverse septa only is characteristic of:
• The type of sporulation of the dematiaceous mold that is illustrated in this photomicrograph is called:
• Of the following dematiaceous fungi, the black, suede-like colony illustrated here, reaching no larger than the size of a dime after 7 days incubation...
• The dematiaceous colony illustrated here grew to a diameter of 3 - 4 cm in 5 days. The dematiaceous fungus that can be ruled out is:
• The dematiaceous fungus that may produce both acrotheca and rhinocladiella types of sporulation is:
• Saprophytic Cladosporium species may be difficult to differentiate from Cladosporium trichoides (Xylohypha bantianum) in culture as both produce chain...
• The black yeast colony illustrated in this photograph may represent any of the following dematiaceous molds except:
• The chain of conidia illustrated in this photomicrograph, with the deep-staining truncated bases, are called annelloconidia, and are most characterist...
• In this photomicrograph are observed several background dematiaceous hyphae within which is seen a long, flask-shaped, tapered phialide (arrow) that h...
• The disease with which the dematiaceous fungus illustrated in this photomicrograph is most likely associated is:
• The dematiaceous conidium illustrated in this photomicrograph was obtained from a tiny portion of dark colony that grew to maturity in  six days....
• The chief microscopic feature possessed by Ulocladium species by which it can be differentiated from the close look-alike Stemphilium species is:
• The etiologic agent of the superficial skin infection tinea niger palmaris (plantaris) is:

Additional Information