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Monomer Information and Courses from MediaLab, Inc.

These are the MediaLab courses that cover Monomer and links to relevant pages within the course.

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CLIA Hematology / Hemostasis Review
A normal hemoglobin molecule is comprised of the following:View Page
Which changes Fibrinogen into Fibrin Monomer:View Page

Fundamentals of Hemostasis
Overview of Secondary Hemostasis

Secondary hemostasis is the series of interrelated chemical processes which lead to the formation of durable fibrin strands, as well as being involved in their incorporation into the existing platelet plug, creating a fibrin clot. The fibrin strands themselves are manufactured through the interaction of various coagulation factors, via a process known as the coagulation cascade. After strand construction, these fibrin monomers are woven into the framework of the platelet plug, adding greater strength and stability. Once woven into the platelet plug, and further stabilized with covalent cross-linking, a fibrin clot (the end goal of secondary hemostasis) is achieved. The fibrin clot is more durable than the platelet plug, and is more of a long term fix, allowing time for continued vascular repair.

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Secondary Hemostasis – The Common Pathway

Thrombin, after its conversion from prothrombin, catalyzes the conversion of fibrinogen into a fibrin monomer. Additionally, thrombin triggers the conversion of factor XIII into factor XIIIa which forms covalent bonds that crosslink and stabilize the fibrin monomers. Finally, thrombin feeds back into the intrinsic and common pathways, accelerating the action of factors XI, V, and VIII.

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Fundamentals of Molecular Diagnostics
Terms and Definitions

Term Definition Codon A three nucleotide base sequence that codes for an amino acid Genome The genetic code composed of 64 codons that code for 21 amino acids and 3 stop codons. (amino acids are the building blocks of proteins and stop codons stop the writing process much like a period at the end of a sentence) Nucleic acid Polymer made of monomers; two examples are RNA and DNA Transcription Process of transferring information from DNA into an RNA message Translation The formation of an amino acid from RNA Deoxyribonucleic Acid (DNA) A double-stranded polymer of nucleotides that houses genetic information Ribonucleic acid (RNA) Typically a single-stranded polymer that is much shorter than DNA but chemically similar with a few differences (e.g.- uracil replaces thymine). Replication Reproduction of DNA content from parent to daughter cell during cell division Amplification methods Techniques that increase the amount of the target, the detection signal, or the probe so that sequences are readily detected Fluorescence The emission of light at a longer wavelength when the light is excited at a shorter wavelength Oligonucleotide Short single-stranded nucleic acid Probe A nucleic acid used to identify a hybridization target Polymerase Chain Reaction (PCR) An amplification method performed in vitro

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