Molds Information and Courses from MediaLab, Inc.
These are the MediaLab courses that cover Molds and links to relevant pages within the course.
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|Which of the following is NOT a tool or instrument utilized for routine paraffin embedding? ||View Page|
Different types of base molds may be used to provide a "frame" for arrangement of the specimen and to hold the molten paraffin while it hardens. The most common type is the metal base mold, but other types such as plastic disposable molds, may be used. The outer dimensions of base molds are standardized to fit within the sizes of most manufacturers' microtome block holders. The block face (indicated by the arrows) is the centrally located, more deeply recessed surface where the tissue specimen will be placed and arranged. This recessed area, which becomes the block face, can be different sizes (shown in the image) to accept differently sized specimens. The base mold size selected should be based on the specimen type and size with consideration of the microtomy protocol that is expected to be performed for that specimen type.
Good practices and careful attention can prevent contamination from block to block from occurring during the embedding step.Open only ONE cassette at a time. Open lids, mesh bags, and lens papers carefully to avoid specimen fragments from "flipping" outward and potentially being lost. Wipe forceps between each specimen. Be especially careful with forceps bearing small "teeth" or grooves at the tips. Wash non-disposable base molds frequently. It is good practice to wipe a mold that has previously been used with a gauze pad before using for another specimen.Embed any pieces that appear to "not belong" or to be contamination from grossing or handling into a far corner of the block face away from what you believe to be the intended specimen. This communicates that you think it "doesn't belong" and makes it easy to identify/locate for removal from the block if it is determined that it is a contamination.
|The cells marked by blue arrows in the image below are associated with all of the following conditions except:||View Page|