Lysed Information and Courses from MediaLab, Inc.
These are the MediaLab courses that cover Lysed and links to relevant pages within the course.
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| An Example of Xanthochromia Two to four hours after a subarachnoid hemorrhage, the supernatant of a CSF sample will be pale pink to pale orange.
The source of this color is oxyhemoglobin from lysed red cells present in the CSF before the puncture. Xanthochromia from the lysed red cells reaches its peak 24 - 36 hours after the hemorrhage and gradually disappears after four to eight days.
In the same type of hemorrhage, after 12 hours yellow xanthochromia begins to appear due to the presence of bilirubin. The bilirubin is the breakdown product of oxyhemoglobin from the original lysed red cells.
The yellow color in the supernatant reaches its peak in about two to four days and disappears after two to four weeks. | View Page |
| More on Undiluted Specimens In an undiluted specimen, count and differentiate red cells and white cells at the same time. You can count red cells on a hand counter and use the differential counter for white cells.
If you cannot differentiate white cells from red cells in the undiluted specimen, a plain capillary tube may be filled with crystal violet acetic acid diluent which is subsequently expelled from the tube. A very thin coating of the diluent will remain on the inside of the tube. CSF is drawn halfway up into the tube, which is then rocked back and forth to mix. The hemacytometer is then filled with the fluid containing stained white blood cells and lysed red cells.
If cells are numerous and overlapping and it is necessary to focus through several planes in order to see all of the cells, a dilution must be made.
When macroscopic appearance is turbid, milky or bloody, a significant dilution is usually necessary. | View Page |
| Leukocyte Esterase Dipstick Test If leukocyte esterase is detected, a color change occurs on the reagent pad after the strip is dipped in the urine sample. Be sure to follow the manufacturer's directions for read-time and test interpretation. A positive leukocyte esterase test indicates the presence of granulocytic white blood cells. Lymphocytes do not contain granules, and would not produce a positive leukocyte esterase test. Positive results should be confirmed by performing a microscopic examination on the sediment; being aware that white blood cells may be absent if they are lysed, yet releasing their esterases into the specimen. Positive results may occasionally be found in random specimens from females due to contamination of the specimen by vaginal discharge. | View Page |
| Laboratory Tests of Hemostatic Function Specimen rejection criteria established by your laboratory should be followed at all times, as improperly collected or processed coagulation specimens could adversely affect patient results. Generally speaking, hemolyzed specimens should not be used in coagulation testing because ADP liberated from lysed red blood cells can interfere with a number of coagulation tests, especially those involving platelet assessment. Grossly lipemic specimens may cause erroneous results or a clot may not be detected if a photo-optical coagulation system is used. An alternative method that is not affected by lipemia, such as an electromechanical method, may be required One way to avoid a grossly lipemic specimen is to ask the patient to fast prior to specimen collection. | View Page |
| Lysis of Red Blood Cells with Acetic Acid This slide shows residual yeast after red blood cells have been lysed with acetic acid. | View Page |