Ligase Information and Courses from MediaLab, Inc.
These are the MediaLab courses that cover Ligase and links to relevant pages within the course.
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| Amplified Nucleic Acid Testing Some methodologies include: Polymerase Chain Reaction (PCR) Ligase Chain Reaction (LCR) Transcription Mediated (TMA) Transcription Based (TAS) Strand Displacement (SDA) Branched DNA (bDNA) Loop mediated (LAMP) Nucleic acid sequence based (NASBA) | View Page |
| Amplified Nucleic Acid Testing Principle These methodologies use principles that amplify or multiply the target of interest, usually incorporating an enzyme to produce millions or billions of copies in a relatively short time.Some enzymes used in amplification include: DNA ligase DNA polymerase RNA polymerase Reverse transcriptase Alkaline phosphatase Cleavase Note: the steps in amplified testing will vary depending on the target amplified, requirement for thermal cycling and detection techniques. | View Page |
| Amplified Nucleic Acid Tests Amplification Method Amplifies Use of Thermal Cycling (Thermocycling) Polymerase Chain Reaction (PCR) Target amplification using DNA polymerase Yes Ligase Chain Reaction (LCR) Target amplification using DNA ligase Yes Transcription- based or Transcription-mediated amplification(TMA) Target amplification using reverse transcriptase and RNA polymerase No Strand Displacement (SDA) Target amplification using DNA polymerase that continuously displaces strands of DNA containing the target sequence No Branched DNA (bDNA) Signal amplification using alkaline phosphatase No Loop Mediated (LAMP) Target amplification of multiple DNA sequences in a loop pattern using DNA polymerase No Nucleic acid sequence based (NASBA) Target amplification using 3 enzymes No Q-beta Replicase Probe amplification- The concentration of an RNA probe increases if the target is present No | View Page |
| Match the following tests to their appropriate principle: | View Page |