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Lactophenol Information and Courses from MediaLab, Inc.

These are the MediaLab courses that cover Lactophenol and links to relevant pages within the course.

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Mycology: Hyaline and Dematiaceous Fungi
A dull white fungus, turning mouse gray on maturity, was recovered from material aspirated from a bone cyst in the upper femur. Based on the microscopic appearance as seen in a lactophenol blue mount of a portion of the colony, the most likely identification is:View Page
The fungus illustrated in this photomicrograph of a lactophenol blue-stained preparation was recovered from skin scrapings of a patient with tinea pedis. The most likely identification is:View Page

Mycology: Yeasts and Dimorphic Pathogens
The colonies shown in the upper image were obtained on blood agar from a sputum specimen after 10 days incubation at 30°C. The lower image is a photomicrograph of a lactophenol blue mount made from a portion of the colony. The diagnosis is:View Page
The growth of the colonies shown in the upper image was obtained on blood agar from a sputum specimen after 8 days of incubation at 30°C. The lower image is a photomicrograph of a lactophenol blue mount made from a portion of the colony. The diagnosis is:View Page
The colonies shown in the upper image, obtained from a biopsy of an ulcerating skin lesion of the arm, are growing on agar slants of Sabouraud's dextrose agar. The lower image is a photomicrograph of a lactophenol blue mount made from a portion of the colony growing in the left slant. The diagnosis is:View Page
One of the characteristics common to the dimorphic molds is the ability to convert the mold forms to the yeast forms by incubating subcultures in enriched media at 35°-37°C. The upper image illustrates a subculture of a mold colony suspected of being a dimorphic fungus inoculated to the surface of blood agar and incubated for 3 days at 37°C. Note that the colonies have a prickly appearance, suggesting an intermediate stage of conversion. The lower image is a lactophenol blue mount of a portion of one of the prickly colonies. This fungus can be identified as:View Page
This image illustrates a lactophenol blue mount of a mold recovered after 7 days incubation of brain heart infusion broth. The large macroconidia suggests the mold form of Histoplasma capsulatum. However, there is the possibility that this mold represents its saprophytic counterpart, which is:View Page
This image illustrates a lactophenol blue mount prepared from a mold recovered after 7 days incubation on brain heart infusion broth. The individual microconidia, each borne by a delicate conidiophore, suggests the mold form of Blastomyces dermatitidis. However, there is the possibility that this mold represents its saprophytic counterpart, which is called:View Page
The growth of the yeast-like colonies shown in the upper image was obtained on blood agar from a skin culture only in the area overlaid by virgin olive oil. The lower image is a photomicrograph of a lactophenol blue mount made from a portion of the colony. The disease associated with this fungus is:View Page
Illustrated in this photomicrograph of a lactophenol blue preparation of a urine sediment is a cluster of yeast cells that were presumptively identified as Cryptococcus species. Further characteristics that may assist in confirming this identification are:View Page


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