Introduction to Bone Marrow (Online Course)
John R. Snyder, Ph.D., MT(ASCP), S.H.; Marjorie A. Spahn, MT(ASCP)
This introductory course about bone marrow examination includes basics of specimen collection, specimen preparation, cellularity, evaluation of iron smears, Romanowsky stained smears, and how to perform a differential count. Unusual cells such as sea blue histocytes, flame cells, and Gaucher cells are covered. This course is a great introduction to cross train medical technologists prior to assisting physicians with marrow preparation and counting. It would also be appropriate for clinical laboratory science students.
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Continuing Education Credits
Objectives
- Explain why a bone marrow examination may be needed.
- Give examples of disorders in which a bone marrow evaluation may be needed.
- Understand how to evaluate bone marrow cellularity.
- Be able to evaluate bone marrow iron stains.
- Understand how to perform a differential count on Romanowsky stained bone marrow smears.
Course Outline
Click on a link in the outline to view a sample page from this course.
- Introduction to Bone Marrow Evaluation
- Bone Marrow Structure
- Basic Structure and Function of Bone Marrow
- Basic Structure and Function of Bone Marrow Cont'd
- Sinuses/Blood Vessels
- Cellular Componenets of Hematopoietic Cords
- Supporting Cells
- Fat Cells
- Location of Cells within Cord
- Location of Cells within Cord continued
- Lymphocytes
- Changes in Cell Distribution
- Summary
- The bone marrow is one of the largest organs in the body.
- The bone marrow begins to produce blood cells in the ________ month of gestation.
- Which of the following bones are active cell-producing sites throughout life? (Choose ALL of the cor
- Which of the following statements are true for the blood vessel/sinus compartment of the bone marrow
- Venous sinuses alternate with hematopoietic cords in a spokelike pattern.
- The cells that provide support for the developing cells in the hematopoietic cords are:
- The average bone marrow cellularity in a normal adult is:
- The young cells that can be found surrounding a macrophage are:
- Collection and Preparation of Bone Marrow
- Bone Marrow Samples
- Collection Sites
- Sample Collection
- Prepare the Patient
- Collection of the Aspirate
- Preparation of Direct Smears
- Preparation of Particle Smears
- Preparation of Concentrated Smears
- Flow Cytometry and Cytogenetics
- Collection of Bone Marrow Biopsy
- Biopsy Touch Preparation
- Fixation and Processing of the Biopsy Specimen
- Advantages of a Biopsy Specimen
- The most common site for bone marrow biopsy and aspiration in adults is:
- Which of the following statements are TRUE regarding biopsy specimens? (Choose ALL of the correct an
- Which of the following statements are TRUE for aspirated specimens?
- Match the phrase with its description.
- Evaluation of Marrow Cellularity
- Microscopic Evaluation of Marrow Smears
- Evaluating Cellularity
- Low Power View of Biopsy
- Medium Power View of Biopsy
- Hypocellular Bone Marrow Biopsy
- Hypercellular Bone Marrow Biopsy
- Hypercellular Bone Marrow Biopsy
- Hypoplasia and Hyperplasia
- Match the following:
- Select the correct answer from the choices provided.
- Select the correct answer from the choices provided.
- The peripheral blood red cell count in this patient will likely be:
- The peripheral blood leukocyte count in this patient will likely be:
- The peripheral blood platelet count in this patient will likely be:
- Example
- Evaluation of Marrow Iron
- Biopsy Section and Bone Marrow Smear
- Perl's Prussian Blue Stain
- Iron Storage Site
- Low Power View of Prussian Blue Stained Biopsy
- Another Biopsy Showing Normal Iron Stores
- Absence of Stainable Iron
- Increase Marrow Iron Stores
- Ring Sideroblasts
- Evaluation of Romanowsky Stained Smears
- Romanowsky Stain
- Examination of Wright-Giemsa Stained Bone Marrow
- Low Power Magnification
- High Power Magnification
- Megakaryocyte Morphology
- Megakaryoblast
- Promegakaryocyte
- Nuclear Division in Megakaryocytes
- Megakaryocyte
- Meta Megakaryocyte Stage
- Another Meta-Megakaryocyte
- Bare Nucleus Megakaryocyte.
- Other Large Cells
- Osteoclasts
- Osteoblast
- More Osteoblasts
- Tumor Cells
- Another Example of Tumor Cells
- Please identify the cell in this image.
- Please identify the cell in this image?
- Alcohol is considered a:
- Please identify the cells present in this image?
- High Power Examination
- Normal M:E Ratio
- Percentages of Myeloid and Erythroid Precursors
- Normal M:E Ratio
- Differentiating Myeloid from Erythroid Cells
- Estimating Myeloid to Erythroid Ratio
- Normal M:E Ratio
- Increased M:E Ratio
- Another Example of Increased M:E Ratio
- Decreased M:E Ratio
- Another Example of Decreased M:E Ratio
- Evaluating M:E Ratio in a Patient with Chronic Lymphocytic Leukemia.
- What is the M:E ratio in this slide?
- What is the M:E ratio in this slide?
- What is the M:E ratio in this slide?
- Representative Counting Field
- Oil Immersion Field
- Macrophage
- Plasma Cells
- Mast Cells
- Increased Plasma Cells
- Flame Cells
- Mott Cell
- Gaucher Cell
- Sea Blue Histocyte
- Match the following.
- Match the following.
- Match the following.
Additional Information
Level of Instruction: Basic
Intended Audience: Medical laboratory science students, medical technologists and MLTs requiring review or cross training, pathology residents.
Course Description: This course explains the anatomy of bone marrow pertinent to marrow examination, collection and preparation of marrow, evalulation of marrow cellularity, iron, and evaluation of Romanowsky stains.
About this Course: This course is part of a series of courses adapted for the web by MediaLab Inc. under license from Educational Materials for Health Professionals Inc. Dayton OH, 45420. Copyright EMHP Inc.
Introduction to Bone Marrow Keywords
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