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Inoculation Information and Courses from MediaLab, Inc.

These are the MediaLab courses that cover Inoculation and links to relevant pages within the course.

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Laboratories Individuals

CLIA Microbiology / Serology Review
Koch's postulates include all of the following except:View Page

Current Topics in Clinical Microbiology
The colonies shown in the blood agar (upper) and MacConkey agar (lower) biplate are a 24 hour growth from an aerobic blood culture bottle that became positive at 12 hours after inoculation. The appearance of the colonies on MacConkey agar rules out the following two bacterial species:View Page
The spot test that is helpful in separating Enterococcus species (positive as shown in the photograph) from the viridans streptococci and S. pneumoniae (both negative) is:View Page
Case History

A 63 year old man was seen in the emergency room with the complaints of sudden onset of fever, chills, and abdominal pain, accompanied by mild diarrhea. The blood pressure was 140/84, the pulse rate 82/minute, and the body temperature 39.8C. A blood sample was drawn for a complete blood count, and a blood culture.A second blood culture was drawn from the opposite arm, with 10 ml of blood being placed into each an aerobic and an anaerobic bottle, following customary practice.The complete blood count revealed a hemoglobin of 15.8 mg/dl, a hematocrit of 45%, and a white blood count of 4.2/L. The neutrophils were 39%, lymphocytes 45%, monocytes 10%, eosinophils 4% and basophils 2%. The platelet count was 255/L. The patient was admitted to the hospital for further work-up and empiric antibiotic therapy.Within 24 hours after admission, the body temperature had decreased to 38.2C, although the mild diarrhea persisted.A stool toxin test for Clostridium difficile was negative and neither enteric pathogens nor Campylobacter species were recovered in stool culture after 24 hours incubation. Fecal neutrophils were not seen on direct examination. The anaerobic blood culture became positive 36 hours after inoculation.

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Clostridium Quad Plate

Key reactions for the identification of Clostridium septicum are shown in the two quadrant plates shown in the photograph.Included in the upper photograph are reactions for milk (casein) proteolysis (12 o'clock quadrant), glucose fermentation, DNAse hydrolysis, and starch hydrolysis respectively reading clockwise.The media in the quadrant plate shown in the lower photograph include gelatin hydrolysis (2 o'clock quadrant) and fermentation of each of mannitol, lactose, and rhamnose respectively, reading clockwise.Milk (casein) hydrolysis Glucose fermentation Key reactions for the identification in the upper plate include no proteolysis of milk, fermentation of glucose (yellow red color along the inoculation streak), positive DNAse (reddish clearing around the streak) and negative reaction for starch. Key reactions in the lower plate include hydrolysis of gelatin, fermentation of lactose (yellow pigment), and negative reactions for mannitol and rhamnose (no pigment).Most strains of C. perfringens hydrolyze starch and produce proteolysins of milk, the key reactions that distinguish C. septicum (negative). Reactions to the other tests do not distinguish between the two.

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Mycology: Yeasts and Dimorphic Pathogens
Although care should be taken when working with all fungus cultures in the laboratory, personnel are particularly prone to develop laboratory acquired infections from the inhalation of airborne species of:View Page
Match the names of each of the yeast species listed with its corresponding appearance when grown in cornmeal agar, as seen in the images.View Page

OSHA Bloodborne Pathogens (updated October 2008)
Transmission of the hepatitis B virus (HBV) can occur from all of the following EXCEPT:View Page

Parasitology Review
Although not always the most practical, animal inoculation may be used to identify which of the following conditions?View Page


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