Inoculated Information and Courses from MediaLab, Inc.
These are the MediaLab courses that cover Inoculated and links to relevant pages within the course.
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| With regard to blood cultures, which blood to broth ratio is most conducive to growth: | View Page |
| Which of the following is the most suitable specimen for the isolation of Bordetella pertussis: | View Page |
| The patient was admitted to the hospital. The sputum specimen was inoculated to sheep blood agar. Based on the colony morphology and the alpha hemolysis seen in the accompanying photograph, the most likely identification is: | View Page |
| Illustrated in this photograph is a quadrant plate containing brain heart infusion agar plate supplemented with 6 ug/ml of vancomycin. The right upper quadrant was inoculated with the test strain of Enterococcus faecium. The presence of growth in the inoculated quadrant indicates resistance to vancomycin. | View Page |
| MRSA Screen Perhaps the most efficient means for detecting methicillin-resistant staphylococci in clinical laboratories is the use of the agar dilution screening test.Illustrated in the photograph is a Mueller-Hinton agar plate containing 6ug/ml of oxicillin, previously inoculated with a strain of Staphylococcus aureus. Oxacillin is used as a marker for methicillin resistance because it is more stable in the agar medium. Growth on this screening medium is presumptive for methicillin resistance.Thus, in the presence of growth, as shown here, a follow-up MIC test must be performed to determine the exact level of resistance. | View Page |
| MRSA Disk Test The disk diffusion test can also be used in the detection of methicillin-resistant Staphylococcus aureus.Illustrated in the photograph is the surface of a Mueller-Hinton agar plate previously inoculated with a strain of S. aureus suspected of being methicillin-resistant. Although the zone of inhibition is at the borderline for resistance (18 mm); the presence of small colonies within the zone of inhibition (yellow arrows) indicates the presence of heteroresistant strains.The interpretation here, therefore, is "methicillin-resistant" staphylococci, even though the zone diameter appears to be adequate. The detection of the heteroresistant strains indicates that MIC studies are required. | View Page |
| Match the names of each of the fungi listed with its appropriate category indicating the degree of pathogenicity. | View Page |
| The disease with which the dematiaceous fungus illustrated in this photomicrograph is most likely associated is: | View Page |
| One of the characteristics common to the dimorphic molds is the ability to convert the mold forms to the yeast forms by incubating subcultures in enriched media at 35°-37°C. The upper image illustrates a subculture of a mold colony suspected of being a dimorphic fungus inoculated to the surface of blood agar and incubated for 3 days at 37°C. Note that the colonies have a prickly appearance, suggesting an intermediate stage of conversion. The lower image is a lactophenol blue mount of a portion of one of the prickly colonies. This fungus can be identified as: | View Page |
| Although not always the most practical, animal inoculation may be used to identify which of the following conditions? | View Page |