Inject Information and Courses from MediaLab, Inc.
These are the MediaLab courses that cover Inject and links to relevant pages within the course.
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| RhIg Policies for Weak D Of the main D variants, a female with partial D (or partial weak D) may develop anti-D to the D epitopes that she lacks. Moreover, partial D red cells adsorb little anti-D, thus leaving enough free RhIg to suppress immunization.However, most laboratories do not routinely differentiate between D variants and instead rely on routine tests and associated test protocols to determine a mother's Rh status for the purposes of RhIg administration. Policies differ between countries and even within countries. 1. Some labs do not test pregnant women for weak D and rely on routine D typing to determine Rh status. AABB Standards for Blood Banks and Transfusion Services, ed. 26 (2010) does not require weak D testing for Rh negative pregnant women, nor for patients requiring transfusion. Note, however, that College of American Pathologists (CAP) surveys have shown that more than 50% of responding laboratories do routinely perform weak D testing on such patients.2. Some labs perform weak D tests on pregnant women who appear to be Rh negative and, if weak D, do NOT inject with RhIg.3. Some labs perform weak D tests on pregnant women who appear to be Rh negative and, if weak D, inject with RhIg based on the possibility that they may be partial D and capable of forming anti-D. | View Page |
| Calculating RhIg Dosage Using the estimated volume of fetal bleed determined by the Kleihauer-Betke test or flow cytometry, the number of vials of RhIg (300 µg) to inject is calculated as follows: Number of vials of 300 µg RhIg = volume of fetal bleed/30 mLIn the interests of safety some American organizations recommend the following to deal with decimal points: If the number to the right of the decimal point is <5, round down and add 1 vial (e.g., 1.4 = 1 +1 = 2 vials) If the number to the right of the decimal point is greater than or equal to 5, round up and add 1 vial (e.g., 1.7 = 2 +1 = 3 vials) Sub-calculations: Volume of fetal bleed: % fetal cells x maternal blood volume Maternal blood volume: 70 mL/kg x weight (kg) (assume 5,000 mL if maternal information is unknown) Note: RhIg dose calculators are available (see Further Reading: Paxton A. Bringing new rigor to RhIG calculations). | View Page |
| The appropriate dosage of Rh immune globulin (RhIg) to administer post-delivery to an Rh-negative mother delivering an Rh-positive child is calculated based on the estimated volume of fetal bleed.What is the value of x in the formula given below that is used to calculate RhIg dosage?Number of vials of 300 µg RhIg = volume of fetal bleed/x mLEnter the number in the box below that is represented by x in the formula; do not spell out the number.(e.g., use "5" and not "five"). | View Page |
| RhIg Policies for Weak D Of the main D variants, a female with partial D (or partial weak D) may develop anti-D to the D epitopes that she lacks. Fortunately, partial D red cells adsorb little anti-D, thus leaving enough free RhIg to suppress immunization.However, most laboratories do not routinely differentiate between D variants and instead rely on routine tests and associated test protocols to determine a mother's Rh status for the purposes of RhIg administration. Policies differ between countries and even within countries. 1. Some labs do not test pregnant women for weak D and rely on routine D typing to determine Rh status. AABB Standards for Blood Banks and Transfusion Services, ed. 26 (2010) does not require weak D testing for Rh negative women.2. Some labs perform weak D tests on pregnant women who appear to be Rh negative and, if weak D, do NOT inject with RhIg.3. Some labs perform weak D tests on pregnant women who appear to be Rh negative and, if weak D, inject with RhIg based on the possibility that they may be partial D and capable of forming anti-D. | View Page |
| Calculating RhIg Dosage Using the estimated volume of fetal bleed determined by the Kleihauer-Betke test or flow cytometry, the number of vials of RhIg (300 µg) to inject is calculated as follows: Number of vials of 300 µg (1500 IU) RhIg = volume of fetal bleed/30 mLIn the interests of safety some organizations recommend the following to deal with decimal points: If the number to the right of the decimal point is <5, round down and add 1 vial (e.g., 1.4 = 1 +1 = 2 vials) If the number to the right of the decimal point is greater than or equal to 5, round up and add 1 vial (e.g., 1.7 = 2 +1 = 3 vials) Sub-calculations: Volume of fetal bleed: % fetal cells x maternal blood volume Maternal blood volume: 70 mL/kg x weight (kg) (assume 5,000 mL if maternal information is unknown) Note: RhIg dose calculators are available (see Further Reading: "Bringing new rigor to RhIG calculations"). | View Page |
