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Indoxyl Information and Courses from MediaLab, Inc.

These are the MediaLab courses that cover Indoxyl and links to relevant pages within the course.

Learn more about laboratory continuing education for medical technologists to earn CE credit for AMT, ASCP, NCA, and state license renewal and recertification. Or get information about laboratory safety and compliance courses that deliver cost-effective OSHA safety training and continuing education to your laboratory's employees.

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Chemical Screening of Urine by Reagent Strip
False Negative Results

False negative bilirubin dipstick results are often due to testing a specimen that is not fresh. Bilirubin breaks down when exposed to light. Indoxyl sulfate (Indican) can produce a yellow orange-to-red color response which may interfere with the interpretation of a positive or negative reaction. Positive nitrites due to a urinary tract infection may also cause a false negative result.

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Granulocytic white blood cells

Granulocytic white blood cells in a urine sample suggest the presence of a urinary tract infection. Granulocytes, which include neutrophils, basophils and eosinophils, contain esterases. These esterases catalyze the strip reagent indoxylcarbonic acid ester to release indoxyl. Indoxyl reacts with a diazonium salt to produce a purple color. The intensity of the color produced is proportional to the amount of enzyme present.

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