Indole Information and Courses from MediaLab, Inc.
These are the MediaLab courses that cover Indole and links to relevant pages within the course.
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|The reactions seen in the portion of the API strip shown in the photograph, effectively rules out Escherichia coli.||View Page|
Although not performed that often, the following tests are useful in separating E. corrodens from other closely related members of the HACEK group: KIA showing an alk/alk reaction; Glucose fermentation (-); Reduction of nitrates to nitrites (+); Production of indole (-); Ornithine decarboxylase (+) The positive nitrate reduction reaction eliminates Cardiobacterium hominis, Kingella kingae and other Kingella species. The positive ornithine decarboxylase reaction eliminates Kingella denitrificans (which also denitrifies nitrate to nitrogen gas, a reaction negative for E. corrodens). Eikenella corrodens is asaccharolytic, whereas most other closely related species produce acid from one or more carbohydrates.
|The indole test may be used to differentiate members of which of the following species:||View Page|
Burkholderia species is a dangerous and highly virulent organism that can cause laboratory-acquired infections. It should NOT be manipulated on an open bench.Catalase: Both organisms are catalase positive. Catalase testing MUST be performed with extreme caution in a biosafety cabinet (BSC) due to the creation of aerosols. Oxidase: B. mallei: Oxidase variable B. pseudomallei: Oxidase positiveIndole: Both organisms are indole negativeMotility: B. mallei: Non-motile B. pseudomallei: Motile
|Which of the following is NOT a characteristic of Burkholderia pseudomallei?||View Page|
Any isolate with the following features should be immediately referred to your LRN reference laboratory: Gram stain shows fat, gram-negative rods in single or short chains that may demonstrate bipolar staining Faster growth at 25oC Gray-white, translucent colonies on sheep blood agar (SBA) at 24 hours that turn slightly yellow and opaque at 48 hours Irregular colonies that have a "fried egg" and/or "hammered copper" appearance after 48-72 hours Catalase positive Oxidase negative Urea negative Indole negative
Any isolate with the following features should be immediately referred to your LRN reference laboratory:B. mallei: Gram stain that reveals pale staining straight or slightly curved gram-negative coccobacilli Cells arranged in end-to-end pairs, parallel bundles, or Chinese letter form Smooth, gray, translucent colonies on sheep blood agar (SBA) at 48 hours Catalase positive Oxidase variable Indole negative Non-motileB. pseudomallei: Gram stain shows slender gram-negative rods with bipolar staining Smooth form appears in Gram stain as long parallel bundles Rough form appears in Gram stain in an irregular arrangement Smooth, creamy, white colonies on SBA at 24 hours Dry, wrinkled colonies at 48-72 hours Catalase positive Oxidase positive Indole negative Motility positive