Immunofixation Information and Courses from MediaLab, Inc.
These are the MediaLab courses that cover Immunofixation and links to relevant pages within the course.
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| Which of the following methods would be used to confirm the presence of Bence-Jones
protein in the urine: | View Page |
| Bence-Jones proteinuria can be seen in all of the following conditions except: | View Page |
| Immunochemical Electrophoresis There are several immunochemical electrophoresis methods used to investigate protein antigens and antibodies in serum. Two methods will be discussed: Immunofixation electrophoresis (IFE) Electroimmunoassay electrophoresis | View Page |
| Immunofixation Electrophoresis An agarose gel electrophoresis first separates the proteins in a serum sample. Antiserum against the protein of interest is spread directly on the gel. The protein of interest precipitates in the gel matrix. After a wash step to remove other proteins, the precipitated protein is stained. This method is qualitative and is used to identify proteins found in multiple myeloma.Below is the immunofixation electrophoresis gel from a serum sample analyzed on SPIFE 3000, Helena Laboratories. After electrophoresis, the precipitated proteins are stained with Acid Violet, a stain developed and used by Helena Laboratories. The SP lane represents a routine serum protein electrophoresis of this specimen. On the next three protein separations, antiserum against IgG, IgA, and IgM were applied to the G, A, M lanes respectively. Antiserum to kappa light chain was added to the next protein separation and antiserum to lambda light chain to the last protein separation. | View Page |