Ictotest Information and Courses from MediaLab, Inc.

These are the MediaLab courses that cover Ictotest and links to relevant pages within the course.

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Chemical Screening of Urine by Reagent Strip
Confirmatory Testing Confirmatory testing using an alternative method such as Ictotest reagent tablets can be performed when positive results are seen on the dipstick strip, when a red color forms on the strip, or when atypical color changes occur that are the result of bilirubin-derived bile pigments in the urine masking the bilirubin reaction.	View Page
A test that can be used as a confirmatory test for urobilinogen is:	View Page

Confirmatory and Secondary Urinalysis Screening Tests
Which of the following would be interpreted as a positive Ictotest®?	View Page
A urine specimen to be tested for bilirubin arrives in the laboratory after sitting on the counter at the nurses station for 2 hours. Which of the following statements describes the Ictotest® reaction that could potentially occur in this situation?	View Page
The Ictotest® Procedure Supplies needed to perform the Ictotest® include the Ictotest® reagent pad, reagent tablet, water and the urine specimen.	View Page
The Ictotest® False positive results can occur in screening procedures for bilirubin due to color interference from large amounts of blood in the urine, very concentrated urine or drugs that discolor the urine such as Pyridium. Because of this it is important to verify positive bilirubin results with a confirmatory test such as the Ictotest®.	View Page
Reporting Ictotest® Results Since detectable amounts of bilirubin are not normally present in urine, results of the Ictotest® are reported as “positive” or “negative”, there is no quantitation. The sensitivity of Ictotest® is better than dipstick methods or the Harrison test. Ictotest® will detect as little as 0.05-0.10 mg bilirubin/dl urine, making it the procedure of choice for confirming bilirubin in urine specimens.	View Page
Principle of the Ictotest® The reaction for the Ictotest® is based on combining a solid diazonium salt with bilirubin. When bilirubin is present, the reaction results in a blue or purple color. No other color change is considered positive.	View Page
The principle of the Ictotest® for bilirubin is based upon:	View Page
Interpret the following Ictotest® results:	View Page
Limitations of the Procedure The product profile for Ictotest® points out that bilirubin is very light sensitive, so urine specimens should be protected from excessive light exposure and examined as quickly as possible when received in the laboratory. On standing, bilirubin, which has a goldish color, is oxidized to biliverdin, which is a green color. Many of the procedures used to detect bilirubin will not react with biliverdin, so false-negative results may occur if urine is not fresh when tested.	View Page
Which of the following may cause a false positive Ictotest®?	View Page
Interpret the following Ictotest® results:	View Page