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Iat Information and Courses from MediaLab, Inc.

These are the MediaLab courses that cover Iat and links to relevant pages within the course.

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Laboratories Individuals

The Disappearing Antibody: A Case Study
ABO, Rh and antibody screen

These ABO, Rh, and antibody screen results were obtained by the TS using the blood specimen that was collected prior to starting the emergency transfusion with O Rh-negative RBCs. ABO and Rh typing ABO Forward Group ABO Reverse Group Rh anti-A anti-B A1 cells B cells anti-D 0 0 4+ 4+ 3+ Antibody screen Cells Gel IAT* Screen Cell I 3+ Screen Cell II 2+ Screen Cell III 2+ * IAT = indirect antiglobulin test

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Crossmatch Results

These are the results of the crossmatch that was being performed in the transfusion service laboratory while the patient was receiving the two units of O Rh-negative RBCs. Cells Gel IAT* Donor I** 2+ Donor 2** 2+ Donor 3 3+ Donor 4 3+ Donor 5 2+ Donor 6 3+ * IAT = indirect antiglobulin test ** O Rh-negative RBC (Donors 3 - 6 are O Rh-positive)

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Antibody identification (2 weeks post-transfusion)

Fortunately, the patient's condition stabilized and additional transfusions were not required. Two weeks later, new patient specimens were drawn for antibody studies. Antibody identification results Cell Rh Rhesus Kell Duffy Kidd MNSs P Lewis Lu Results Cell C D E c e Cw K k Kpa Fya Fyb Jka Jkb M N S s P1 Lea Leb Lua Gel IAT* 1 rr 0 0 0 + + 0 0 + 0 + 0 + 0 0 + + + +S + 0 0 1+ 1 2 rr 0 0 0 + + 0 0 + 0 + 0 + + 0 + + + +S + 0 0 w+ 2 3 rr 0 0 0 + + 0 0 + 0 + + 0 + 0 + + 0 + 0 + 0 0 3 4 r"r 0 0 + + + 0 0 + 0 + + 0 + 0 + 0 + + + 0 0 0 4 5 R2R2 0 + + + 0 0 + 0 0 + + + + + 0 + 0 + 0

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Using the guidelines in the Antibody Exclusion Protocol, which antibodies are possible (have not been excluded) using this panel? Select all that apply.Antibody identification results CellRhRhesusKellDuffyKiddMNSsPLewisLuResultsCell CDEceCwKkKpaFyaFybJkaJkbMNSsP1LeaLebLuaGel IAT* 1rr000++00+0+0+00++++S+001+1 2rr000++00+0+0++0++++S+00w+2 3rr000++00+0++0+0++0+0+003 4r"r00+++00+0++0+0+0+++0004 5R2R20+++00+00+++++0+0+0+0w+5 6View Page
DAT change of status

Notice that the patient's DAT is now negative (IAT autocontrol in the panel done 2-weeks post-transfusion is negative). Cell Rh Rhesus Kell Duffy Kidd MNSs P Lewis Lu Results Cell C D E c e Cw K k Kpa Fya Fyb Jka Jkb M N S s P1 Lea Leb Lua Gel IAT* 1 rr 0 0 0 + + 0 0 + 0 + 0 + 0 0 + + + +S + 0 0 1+ 1 2 rr 0 0 0 + + 0 0 + 0 + 0 + + 0 + + + +S + 0 0 w+ 2 3 rr 0 0 0 + + 0 0 + 0 + + 0 + 0 + + 0 + 0 + 0 0 3 4 r"r 0 0 + + + 0 0 + 0 + + 0 + 0 + 0 + + + 0 0 0 4 5 R2R2 0 + + + 0 0 + 0 0 + + + + + 0 + 0 + 0 + 0 w+ 5 6

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Which of the following antibodies in this scenario could explain all reactions by itself?Antibody identification results CellRhRhesusKellDuffyKiddMNSsPLewisLuResultsCell CDEceCwKkKpaFyaFybJkaJkbMNSsP1LeaLebLuaGel IAT* 1rr000++00+0+0+00++++S+001+1 2rr000++00+0+0++0++++S+00w+2 3rr000++00+0++0+0++0+0+003 4r"r00+++00+0++0+0+0+++0004 5R2R20+++00+00+++++0+0+0+0w+5 6R2R20++<View Page


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