Histoplasma Information and Courses from MediaLab, Inc.
These are the MediaLab courses that cover Histoplasma and links to relevant pages within the course.
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| The hyaline saprobic fungus that has microscopic features similar to the mold form of Histoplasma capsulatum is: | View Page |
| Several saprophytic, hyaline molds have microscopic characteristics that mimic the mold forms of the dimorphic fungi (Histoplasma capsulatum, Blastomyces dermatitidis, etc.). Each of the following can be used to differentiate the saprophytic from the dimorphic fungi except: | View Page |
| The dimorphic fungus that may produce black, yeast-like colonies after prolonged incubation at 37°C is: | View Page |
| Match each of the names of the dimorphic fungal species with its corresponding mold form as seen in the photomicrographs. | View Page |
| Match each of the names of the dimorphic fungi listed with the names of the animals that most commonly may be related to transmission of disease to humans. | View Page |
| Arrange the fungal species that are listed in the drop-down box according to length of time of recovery in primary culture; from most rapid to the slowest. | View Page |
| The colonies shown in the upper image were obtained on blood agar from a sputum specimen after 10 days incubation at 30°C. The lower image is a photomicrograph of a lactophenol blue mount made from a portion of the colony. The diagnosis is: | View Page |
| The growth of the colonies shown in the upper image was obtained on blood agar from a sputum specimen after 8 days of incubation at 30°C. The lower image is a photomicrograph of a lactophenol blue mount made from a portion of the colony. The diagnosis is: | View Page |
| The colonies shown in the upper image, obtained from a biopsy of an ulcerating skin lesion of the arm, are growing on agar slants of Sabouraud's dextrose agar. The lower image is a photomicrograph of a lactophenol blue mount made from a portion of the colony growing in the left slant. The diagnosis is: | View Page |
| One of the characteristics common to the dimorphic molds is the ability to convert the mold forms to the yeast forms by incubating subcultures in enriched media at 35°-37°C. The upper image illustrates a subculture of a mold colony suspected of being a dimorphic fungus inoculated to the surface of blood agar and incubated for 3 days at 37°C. Note that the colonies have a prickly appearance, suggesting an intermediate stage of conversion. The lower image is a lactophenol blue mount of a portion of one of the prickly colonies. This fungus can be identified as: | View Page |
| This image illustrates a lactophenol blue mount of a mold recovered after 7 days incubation of brain heart infusion broth. The large macroconidia suggests the mold form of Histoplasma capsulatum. However, there is the possibility that this mold represents its saprophytic counterpart, which is: | View Page |
| This image illustrates a lactophenol blue mount prepared from a mold recovered after 7 days incubation on brain heart infusion broth. The individual microconidia, each borne by a delicate conidiophore, suggests the mold form of Blastomyces dermatitidis. However, there is the possibility that this mold represents its saprophytic counterpart, which is called: | View Page |
| Procedures for the rapid culture confirmation of suspected colonies of B. dermatitidis, C. immitis and H. capsulatum recovered from clinical specimens include: | View Page |
| The colonies growing on the surface of this brain-heart infusion with blood agar plate were "converted" from a mold colony suspected of being Histoplasma capsulatum by incubating a subculture at 37°C for 5 days. The yeast forms that must be identified in mounts made from one of these colonies to confirm the identification are: | View Page |
| Although care should be taken when working with all fungus cultures in the laboratory, personnel are particularly prone to develop laboratory acquired infections from the inhalation of airborne species of: | View Page |
| A hematology technologist observed the intracellular forms seen in the field of view of a Wright-Giemsa-stained peripheral blood smear shown in this photomicrograph. In consultation, the microbiology technologist advised that the form seen most likely represents: | View Page |