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Online compliance and continuing education courses for clinical laboratories

Health Information and Courses from MediaLab, Inc.

These are the MediaLab courses that cover Health and links to relevant pages within the course.

Learn more about laboratory continuing education for medical technologists to earn CE credit for AMT, ASCP, NCA, and state license renewal and recertification. Or get information about laboratory safety and compliance courses that deliver cost-effective OSHA safety training and continuing education to your laboratory's employees.

Laboratories Individuals

Alpha Thalassemia
References

Burtis, CA. & Ashwood, ER. Tietz Textbook of Clinical Chemistry 2nd ed. W. B. Saunders. 1994.Harmening, DM. Clinical Hematology and Fundamentals of Hemostatis 5th ed., F.A. Davis, 2008Lotspeich-Steininger, Stiene-Martin and Koepke, Clinical Hematology Principles, Procedures, Correlations, Lippincott 1992McKenzie, SB., Textbook of Hematology 2nd ed., Williams and Wilkins 1996.Miale, JB, Laboratory Medicine Hematology 6th ed., Mosby 1982.Nouwens, J and Spahn, M. Hemoglobin H Disease: A self-instructional unit 3rd ed., Educational Materials for Health Professionals, Inc. 1991.Doig, K. Rodak's Diagnostic Hematology 3rd ed. W.B.Sunders Co., 2007.

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Cerebrospinal Fluid

Chemical Screening of Urine by Reagent Strip

CLIA General Laboratory Review
In what section of the MSDS manual would you find information on which chemical might affect your health:View Page
Which one of the following does not directly regulate clinical laboratories:View Page
Which of the following infectious agents represent the greatest risk to the laboratory worker:View Page
Which section of the MSDS would provide information on chemical hazards that might affect your health:View Page

Confirmatory and Secondary Urinalysis Screening Tests

Current Topics in Clinical Microbiology
Review 2

Griego RD. Rosen T. Orengo IF. Wolf JE.: Dog, cat, and human bites: a review. Journal of the American Academy of Dermatology. 33:1019-29, 1995It is estimated that half of all Americans will be bitten by an animal or another human being during their lifetimes. The vast majority of the estimated 2 million annual mammalian bite wounds are minor, and the victims never seek medical attention. Nonetheless, bite wounds account for approximately 1% of all emergency department visits and more than $30 million in annual health care costs.Infection is the most common bite-associated complication; the relative risk is determined by the species of the inflicting animal, bite location, host factors, and local wound care. Most infections caused by mammalian bites are polymicrobial, with mixed aerobic and anaerobic species.The clinical presentation and appropriate treatment of infected bite wounds vary according to the causative organisms. Human bite wounds have long had a bad reputation for severe infection and frequent complication. However, recent data demonstrate that human bites occurring anywhere other than the hand present no more of a risk for infection than any other type of mammalian bite.The increased incidence of serious infections and complications associated with human bites to the hand warrants their consideration and management in three different categories: occlusional/simple, clenched fist injuries, and occlusional bites to the hand. This article reviews dogs, cat, and human bite wounds, risk factors for complications, evaluation components, bacteriology, antimicrobial susceptibility patterns, and recommended treatments. Epidemiology, clinical presentation, and treatment of infections caused by Pasteurella multocida, Capnocytophaga canimorsus, Eikenella corrodens, and rhabdovirus (rabies only) receive particular emphasis.

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Department of Transportation (DOT) Regulated Urine Specimen Collection Training
Monitored collection

For monitored collections, the Department of Transportation classifies the following as health professionals: Physician Medical Technologist Medical Laboratory Technician Nurse (RN/LPN) Physician's Assistant/Nurse Practitioner Medical Technician (A medical technician is anyone who is licensed or certified to practice in the institution where the collection is being done. For example, a phlebotomist, EMT, or medical assistant.)

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Monitored collection

Another type of collection is the monitored collection. Monitored collections occur where the collection must take place in a multi-stall restroom with partial length doors.The collector enters the restroom with the donor, but the donor goes into the stall alone and closes the door. It is preferred that the collector be the same sex as the donor, but it is not required so long as the collector is classified as a health professional.

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In both the monitored and observed collections, the collector MUST be the same gender as the donor.View Page

Descriptive Statistics

Erythrocyte Inclusions - Wright Stained Smears

Fundamentals of Hemostasis

Fundamentals of Molecular Diagnostics
Infectious Diseases

Molecular methodologies can be useful in the detection of a variety of diseases that are important public health issues such as:Chlamydia trachomatis (CT) Neisseria gonorrhoeae (GC)Human papillomavirus (HPV)Human Immunodeficiency Virus (HIV)Herpes Simplex Virus (HSV)Cytomegalovirus (CMV)In many clinical laboratories, traditional methods have been replaced by molecular methodologies because testing can occur for several pathogens in a single specimen. This is termed multiplex testing.

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HIPAA Privacy and Security Regulations
What is the HIPAA Security Regulation?

The HIPAA Security Regulation: Defines how to protect electronic health information. Went into effect April 20,2005

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Case Study: Physical SafeguardsYou are a supervisor of a health clinic. During orientation of a new employee, you instruct him to keep the door leading from a patient area to a computer work area locked at all times. On several occasions, he forgets to make sure the door is locked as he leaves. Which of the following are true regarding this situation?View Page
What is the HIPAA Privacy Regulation:

The HIPAA Privacy Regulation went into effect April 14, 2003. It protects the confidentiality of individuals' health data by:Regulating how Protected Health Information (PHI) is used, whom it is disclosed to, and how and where it is maintained. The HIPAA Privacy Regulation:Requires reasonable security measures to protect individuals' health information. Establishes accountability for use and release of this information. Gives individuals rights regarding their health information.

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What Information is Protected?

HIPAA protects ALL information related to an individual's physical or mental health, including demographic and payment information, whether oral, written, or in computer format. All such information is referred to as Protected Health Information or PHI.

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Limiting Use and Disclosure of PHI

A covered entity may use or disclose PHI, without getting an individual's authorization, in order to:Perform requested tests and treatments.Bill for the services performed.Perform essential operations, including quality assessment, accreditation, and compliance.Meet legal reporting requirements, including those mandated by public health departments, workers' compensation, law enforcement agencies, and the US Department of Health and Human Services. Other uses and disclosures require written authorization.

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De-Identified Health Information

Health information is considered de-identified, if it cannot be used to identify an individual. Other terms for de-identified health information are: Anonymous, Aggregated, and Scrubbed.The Privacy Regulation details the specific individual information which needs to be removed from PHI for it to qualify as de-identified.When PHI is de-identified, it is no longer considered protected.De-identified health information should be used whenever individual patient identification is unnecessary.

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Case Study: Minimum Necessary Use & Disclosure You are a phlebotomist at a specimen collection center. A patient arrives with an order for a blood glucose test, and a lipid profile. You get the patient's address, phone number, health insurance coverage, and ask how long ago he ate his most recent meal. You then ask him about his recent auto accident, his wound infection, and his family. You write down all the extra information. Under the HIPAA Privacy Regulations, which of the following information requests is acceptable?View Page
Authorization

The privacy regulations give covered entities permission to use and disclose PHI for treatment, payment, and health care operations (TPO), without obtaining specific authorization.A covered entity may disclose PHI to other covered entities such as reference laboratories, and homecare services, which are providing services to the primary covered entity.The service that the other covered entity is providing must fall within treatment, payment or health care operations (TPO).If the service being provided does not fall within TPO, an authorization is generally required.An authorization form must state the specific disclosures of PHI to be made, what the information will be used for, and must be signed and dated by the patient.

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Case Study: De-identified Health Information. You work in a laboratory microbiology department which provides a local nursing home with information about the effectiveness of various antibiotics it uses to treat infections. You print the requested information, including complete patient identification, bacterial organisms identified, and their sensitivity to various antibiotics. What information should you provide to the nursing home?View Page
Business Associate Agreement

A Business Associate is a separate organization, providing services to a covered entity, which require the exchange of PHI.An agreement must be in place between covered entities and their business associates.This agreement defines the processes that will be implemented to ensure the privacy and security of PHI.Examples of Business Associates may include collection agencies, attorneys, consultants, and accountants, requiring access to PHI.Business Associate agreements are not generally required between two covered entities involved in treatment, payment, or health care operations.

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Privacy is Your Responsibility.

As a health care worker, you are required by HIPAA to protect the privacy and security of the personal health information to which you have access.

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HIPAA Enforcement

The penalties for HIPAA violations are substantial: Fines could range from $100 to $250,000. Knowingly and wrongfully receiving or disclosing protected health information could also result in a maximum 10 year prison sentence.

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Which of the following entities are covered by HIPAA?View Page
Importance of Privacy - An Example

You will have many opportunities to avoid disclosing protected health information. Here is one simple example: Your best friend asks you to look up her mother's laboratory results. Knowing the HIPAA privacy regulation and your own departmental policies and procedures, you do not disclose the protected health information which she is requesting. You politely tell your friend that your are not allowed to give her the laboratory results.

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What is HIPAA?

HIPAA is a federal law passed by Congress in 1996 to help protect the privacy and security of health information. It is short for Health Insurance Portability and Accountability Act.

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Who does HIPAA apply to?

HIPAA applies to: Health Plans (such as health insurance companies) Healthcare Clearinghouses (such as billing companies), and Healthcare Providers (including doctors, hospitals, laboratories, and pharmacies). HIPAA refers to these 3 groups as covered entities.

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HIV Safety for Florida
A person commits a misdemeanor of the first degree by:View Page
Informed Consent

Informed consent must be obtained first before a HIV antigen or antibody test can be ordered.Informed consent must be preceded by: Explanation of the test subject's right of confidentiality. Notification that a positive HIV test result will be reported to county health department with enough information to possibly identify the test subject. Availability and location of sites where anonymous testing is performedInformed consent can be given by a legal guardian or other person authorized by law when the test subject is: not competent incapacitated a minor

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Reporting results

Each laboratory that performs a test indicative of HIV or AIDS shall report to the county health department in less than 2 weeks.To assure the confidentiality of the patient, reporting of HIV infection and AIDS must be conducted using a system developed by CDC or equivalent system.Each person who violates reporting rules may be fined $500 per offense.

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Legislative Intent

The Florida Legislature finds that the public health will be served by facilitating informed, voluntary, and confidential use of tests designed to detect HIV infection.

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References

Panlilo AI, Cardo DM, Grohskopf LA, Heneine W, Ross, CS. Updated U.S. Public Health Service Guidelines for the Management of Occupational Exposures to HIV and Recommendations for Postexposure Prophylaxis, 2005. MMWR Recomm Rep 2005 Sep 30; 54:RR-9.The 2005 Florida Statutes, Chapters 381, 384,456. Available at www.leg.state.fl.us.MediaLab Course "HIV: Structure and Replication," Garland Pendergraph.MediaLab Course "OSHA Blodborne Pathogens," Terry Jo Gile MT(ASCP),Ma Ed.

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Introduction to Bioterrorism
Category A

Those agents in Category A are highest-priority because they: Can be easily disseminated or transmitted person-to-person Cause high mortality, with potential for major public health impact Might cause public panic and social disruption Require special action for public health preparedness

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Category C

Agents in the third highest priority, Category C, include emerging pathogens that could be engineered for mass dissemination at some future date because of: Availability Ease of production and dissemination Potential for high morbidity and mortality and major health impact

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Biological agents that are the easiest to disseminate, or that pass most easily from person to person are found in Category:View Page
Disadvantages of using Biological Agents

They are not immediate. The delayed effect, for example, the long incubation period for some agents, may detract and limit their tactful usefulness as a political statement.They are hazardous to all who come in contact.There is the possibility that the biological agents could also affect the health of the aggressor forces. They are hard to control.The dependence of prevailing winds and other weather conditions such as temperature, sunlight, and desiccation may make it difficult to control distribution of the biological agent.  Potential long term effects beyond the initial attack.The persistence of some agents such as spore-forming anthrax in the environment may make an area uninhabitable to aggressor forces for long periods. Results are unpredictable.Morbidity secondary to a biological attack is unpredictable since casualties will be related to the quantity and manner of exposure plus the preventive and treatment measures available.

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Laboratory Response

The broad base of clinical laboratories in this country is an essential component of our nation’s public health and healthcare system and is an essential link in addressing biological and chemical terrorism. In 1999 the Centers for Disease Control and Prevention (CDC) initiated the concept of a Laboratory Response Network (LRN).  The LRN is a network of local, state, federal, and military laboratories across the United States and internationally which work together in an integrated and coordinated way for a rapid response to public health emergencies. The LRN concept of operations is based on a system of safety and proficiency.

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The LRN Pyramid

The LRN is a multilevel system designed to link frontline clinical microbiology laboratories and hospitals and other institutions to state and local public health laboratories in supporting advanced capacity public health, military, veterinary, agricultural, water and food testing laboratories at the federal level. Laboratories within the LRN are divided into 3 levels: Sentinel Labs, Reference Labs, and National Labs.

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Laboratory Response - Chemical

Currently there are over 60 territorial and metropolitan public health laboratories that are members of the chemical component of the LRN. A designation of Level 1, 2, or 3 defines their network participation.

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Laboratory Response - Chemical, Level 3

Level 3 laboratories are responsible for: Working with hospitals and private laboratories in their jurisdiction Knowing how to properly collect and ship clinical specimens Ensuring that specimens, which can be used as evidence in a criminal investigation, are properly handled and that chain-of-custody procedures are followed Being familiar with chemical  agents and how they can affect health and well-being Training on anticipated clinical sample flow and shipping regulations Working to develop a coordinated response plan for their respective state and jurisdiction

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Sentinel Labs

The frontline clinical microbiology laboratories are known as “sentinel laboratories”. The sentinel laboratories play a key role in the nation’s preparedness efforts. These laboratories perform the initial screening of clinical specimens for potential pathogens (rule-out) and refer specimens or isolates to a state or local public health laboratory at the reference level of the LRN. There are two kinds of sentinel laboratories: advanced and basic. Classification depends on their biological safety level and analytical capability.

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Introduction to Bone Marrow

Introduction to Quality Control
Federal Regulations

The importance of quality control is recognized by the federal government. Federal regulations, such as the Clinical Laboratory Improvement Amendments of 1988 (CLIA 88), require successful participation in external quality control programs for clinical laboratories, regardless of size. These regulations are aimed at providing all citizens with the highest quality of health care and at the same time controlling costs.

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Introduction to the ABO Blood Group System

Laws and Rules of the Florida Board of Clinical Laboratory Personnel
Your Role

As a clinical laboratory worker, your role is vital in the health care process. You provide information to doctors, nurses, and healthcare organizations that is vital to proper patient care. Because your role is so important, you must be properly qualified, trained, and licensed for your position. You must also keep up with the latest laboratory techniques and developments by fulfilling continuing education requirements. And you are bound by a code of ethics, which ensures that patient results are accurate, reliable, and free from error and bias.

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Public health laboratory scientists

Public health laboratory scientists are also regulated by the Board. The table below outlines the various requirements for applicants to receive licensure for a public health laboratory. Public Health Laboratory RequirementsDirectorFulfill the same requirements as a clinical laboratory directorSupervisorBe certified by National Registry in Clinical Chemistry or American Society for MicrobiologyBe licensed as a technologistHave five year's relevant experiencePass the state examTechnician (microbiology)Have a Bachelor's degree in one of the biological sciencesObtain American Society for Microbiology or the National Registry in Microbiology Certification in Public Health Microbiology Technician (chemistry)Have a Bachelor's degree in one of the chemical, biological, or physical sciencesObtain National Registry of Clinical Chemistry Certification in Public Health ChemistryTechnician (conditional)Have a Bachelor's degree in one of the chemical or biological sciencesPerform tests only under the direct supervision of a licensed pathologist, director, supervisor, or technologist.Receives a conditional two-year license, which may be renewed only once A license from the Board of Clinical Laboratory Personnel allows you to work in a public health laboratory at the same level and specialty.

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CEBroker

CEBroker is the Florida Department of Health's online system for tracking continuing education requirements. Companies and colleges that provide continuing education, whether through online programs, lectures, seminars, or other methods, will report your completed credits to CEBroker. All courses you complete through MediaLab that carry Florida credit will be reported to CEBroker and automatically credited to your license. For that reason, make sure you keep your license number up-to-date with MediaLab. Even though CEBroker tracks your credits, keep copies of all Certificates of Completion that you receive in case of an audit. You are responsible for maintaining proper documentation of your completed courses. CEBroker's website is www.cebroker.com.

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Kickback and Inducement Violations

Offering or taking a bribe, kickback, bonus, commission, or inducement is against the rules of the Board and against the law. Many companies give away small promotional items, such as pens or note pads, to promote their products. This is legal, but be cautious about accepting more valuable items. This could be seen as a bribe. All of the following are serious violations of Board, state, and federal rules:Participating in any commissions, bonuses, kickbacks, inducements, or split-fee arrangements from physicians, health care providers, suppliers, hospitals, nursing homes, other clinical laboratories, pharmacies, and other facilities.Exploiting or influencing a patient for financial gain, including promoting, selling, or withholding services, drugs, or referrals.

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Linear Regression Analysis

Medical Error Prevention
Which statement describes an Adverse Event?View Page
Medical Errors in the United States

Most medical interventions produce positive outcomes. Everyone expects to give and receive effective medical care. These expectations are routinely met by the healthcare community. However, a 2000 publication from the Institute of Medicine, the IOM, To err is human: Building a safer health system, reports that medical errors cause as many as 98,000 deaths every year in the United States.

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JCAHO Patient Safety Goals JCAHO adopted national patient safety goals for laboratories and many other healthcare organizations. 2006 Laboratory Services National Patient Safety Goals These goals are directly quoted.View Page
Improving Patient Safety Many other organizations strive to improve patient safety. These dedicated groups promote current information about their goals, projects, progress, publications, tools, and educational offerings in their Internet Websites.View Page
American Society for Clinical Laboratory ScienceThe American Society for Clinical Laboratory Science, ASCLS, joins the leadership effort to prevent medical errors and increase patient safety.View Page
Public Responsibility for Safety

People can help prevent errors in their medical care by understanding their treatment, keeping organized health records, and asking questions. They should feel comfortable talking with medical professionals when things do not seem right.

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Medical Errors

The health community describes medical errors several ways: Failures of planned actionsMistakes of executionUse of wrong plans to achieve outcomes Sometimes peoples' plans and actions fail, and this create medical errors. Sometimes people make mistakes performing their duties, and this creates medical errors. Sometimes people use wrong plans and actions, and this creates medical errors. Medical errors are mistakes medical professionals make in patient testing, care, or treatment.

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Adverse Events

Medical errors lead to adverse events--unintended injuries or other negative health consequences. These unfortunate events are caused by medical mismanagement, not underlying patient diseases or conditions. Adverse events may or may not be preventable.

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Medicare Compliance for Clinical Laboratories
Introduction

The government believes that fraud, abuse and waste exist in the healthcare industry today because of cases it has settled and prosecuted.All healthcare providers, including laboratories, make billing errors.The Office of Inspector General (OIG) believes that honest members of the healthcare community can police themselves if they receive guidance.The OIG has published Compliance Program Guidance documents for health care providers, including laboratories.

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Element 5

Element 5: Every employee must understand that the standards, policies and procedures associated with the compliance program must be adhered to at all times. Employee will be disciplined up to terminations for violations. Employee can be disciplined or terminated for failing to report a problem or suspect activity. All employees are screened prior to being hired for previous actions against them by any law enforcement or government agency regarding any health care prosecution or exclusion from the Medicare or Medicaid program. Adherence to the compliance program's policies and procedures will be a component of every employees annual evaluation and performance review.

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Laws and regulations that govern laboratories

Social Security Act: Medicare and Medicaid laws are in this act. Medicare rules and regulations come under this act. Antikickback laws: Provide criminal penalties for individuals or entities that knowingly and willfully offer, pay, solicit or receive money or favors for referrals of tests or services that will be paid for by the Medicare or Medicaid programs. False Claims Act: Provides criminal penalties for knowingly or willingly filing a false claim to a government program. Self Referral (Stark) laws and regulations: Identify financial relationships that have the potential to result in directed referral to one or both of the individuals or entities involved. Prohibit the referral of patients or tests between related entities unless certain conditions are met. Health Insurance Portability and Accountability Act (HIPAA) Prohibits health care providers and payers from improper or inappropriate use of a patient's confidential health information Requires health care providers to insure that a patient's confidential information is kept secure Provides for standardized electronic formats for all health care transactions

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HCPCS and CPT coding

The HCFA (Health Care Financing Administration) Common Procedural Coding System (HCPCS) and the CPT (Current Procedural Terminology) codes are used to describe specific tests or services. The amount of payment for a test is dependent on the HCPCS or CPT code. HCPCS or CPT codes should be assigned under the supervision of the laboratory technical staff. Billing department employees should never change a HCPCS or CPT code without the approval of a manager or compliance officer. If a billing department clerk notices that a particular HCPCS or CPT code is being rejected by a payer they should report it to their manager. It is against the law to use the wrong HCPCS or CPT code for the purpose of causing or increasing payment for a test.

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Communication with physicians and patients

It is important that billing department employees are clear and accurate when communicating with physician office personnel and patients. Never guess at the answer to a question; ask if you are unsure. Do not speculate or express personal opinions. When requesting diagnosis information from the physician office staff be careful to not lead them to give a billable code: The code must come from the patient's medical record. There is an incentive program for patients to find and report fraud and abuse by health care providers, including laboratories, so: Billing department employees must accurately state laboratory policies and procedures, or forward the call to a supervisor to avoid misstatements and misunderstandings.

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Client contracts

A laboratory that receives referrals from a nursing home or Skilled Nursing Facility (SNF) should have a written agreement with that facility: The Agreement should define billing and documentation responsibilities. The facility should be responsible for determining the payment status of its patients and is liable for submitting incorrect payment information to the laboratory. Fees should be consistent with other similar customers. A laboratory that provides services to a Home Health Agency treating Medicare/Medicaid beneficiaries should have a written agreement with that agency: The Agreement should define billing and documentation responsibilities. The Agreement should place the responsibility on the Home Health Agency to establish that all patients receiving laboratory services are "homebound" as defined by Medicare. Fees should be consistent with other similar customers.

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Normal Peripheral Blood Cells

OSHA Electrical Safety (updated 2007)

OSHA Formaldehyde
A Potential Carcinogen

More importantly, the National Institute for Occupational Safety and Health (NIOSH) has labeled formaldehyde a potential human carcinogen.

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Pharmacology in the Clinical Lab: Therapeutic Drug Monitoring and Pharmacogenomics
Bioavailability

Bioavailability refers to the amount of drug that actually reaches the circulation. It is calculated by comparing (in the same subjects) the area under the serum concentration - time curve (AUC) of an equivalent dose of the intravenous form and oral form. This is illustrated in the diagram on the right.For IV drugs, the bioavailability is 100%For oral medications, the bioavailability will be less than 100%, due in part to any of these reasons:* Oral drugs take longer to enter the circulation.* Oral drugs have slower absorption and distribution than IV drugs.* The amount of drug that is absorbed can depend on the status of the GI tract (stomach pH, presence of food, integrity/health of the intestines, speed of the GI tract, etc.)For oral drugs to be effective, bioavailability typically should be greater than 70%.Not all of a drug taken orally is able to have a pharmacologic effect; the dose would need to be higher than an IV dose.Since the absorption of an oral drug is slower than an IV drug and the drug takes longer to enter the circulation, clearing the drug will also most likely take a longer time.

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Phlebotomy
What is a phlebotomist’s role in a health care facility?

The phlebotomist collects blood & other specimens which ultimately provide doctors and nurses with laboratory test information critical to patient care.He or she therefore plays a vital role in any health care system.

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What is a phlebotomist’s role in health care facility? [continued]

Phlebotomists work in a variety of settings including: Hospitals Physician Offices Nursing Homes Home Health Care Clinics, and Military facilities. A well trained phlebotomist will therefore have a variety of job opportunities available.Other medical professionals, including nurses, respiratory therapists, and medical assistants may also be trained to collect blood specimens.

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What is a phlebotomist?

A phlebotomist is a medical professional who:Collects blood and other specimens.Prepares specimens for testing. Interacts with patients & health care professionals.

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OSHA bloodborne pathogens standard

The Occupational Health and Safety Administration (OSHA), of the federal government has mandated bloodborne pathogen training for all US workers who are at risk of exposure. The next few slides cover a few highlights of this training. You will receive complete OSHA bloodborne pathogens training before you begin work.

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Needlestick safety and prevention act

The Needlestick Safety and Prevention Act was passed by Congress in November 2000. This law modifies the OSHA Bloodborne Pathogens Standard to require that health-care institutions use only needles and other sharps which have engineering controls and design features to help prevent accidental sharps injury. Health care institutions must update their exposure control plans to reflect these changes.

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Reading Gram Stained Direct Smears

Reading Gram Stained Smears From Cultures

Red Cell Disorders: Peripheral Blood Clues to Nonneoplastic Conditions

Red Cell Morphology

Semen Analysis
Sperm count: Normal values

A sperm count is considered normal if it is over 20 million sperm/ml. Although lower counts are considered abnormal by World Health Organization (WHO) standards (see the WHO manual, 1999), it is sometimes possible for men with significantly reduced counts to father a pregnancy.

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The Urine Microscopic: Microscopic Analysis of Urine Sediment

Tuberculosis Awareness for Healthcare Workers
Records and Results

Skin tests are read 48-72 hours after injection by a trained health-care worker such as a physician or nurse.The diameter of any induration is measured in millimeters.Records of the test are kept in the individual's health record for future reference.

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Fundamentals of TB infection control

TB infection controls include: standardized anti-tuberculosis treatment regimens in the initial phase of therapy; rapid drug susceptibility testing; directly observed therapy in which a health professional watches a patient swallow each dose of medication and records the date that the administration was observed; improved infection control practices.

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Biosafety Levels

Laboratory workers who handle infectious materials in the microbiology laboratory should be aware of the work practices, safety equipment, and barriers that will protect them and others in the area from infectious agents. The Centers for Disease Control and Prevention (CDC) and the National Institutes of Health (NIH) created guidelines to assist laboratories in developing safe practices based on the infectious agents that are handled. These guidelines are referred to as Biosafety Levels 1 through 4. Each increasing number represents increased risk, requiring more stringent work practice and increasingly protective safety equipment and barriers. A copy of the Guidelines can be obtained from the CDC or accessed online at:http://www.cdc.gov/OD/ohs/biosfty/bmbl5/bmbl5toc.htm

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Variations in White Cell Morphology - Granulocytes

White Cell and Platelet Disorders: Peripheral Blood Clues to Nonneoplastic Conditions
WBC inclusions: summary

The presence of atypical inclusions within the cytoplasm of neutrophils and other leukocytes should lead to a clinical investigation of the setting for these findings.Atypical neutrophil inclusions may be seen in the following disorders: Chediak-Higashi syndrome, May-Hegglin anomaly, Alder-Reilly anomaly, Fechtner , Sebastian, Epstein and Alport-like syndromes and in infectious and toxic conditions (in the form of Doehle bodies).Although a specific entity may not be evident from examination of the peripheral blood alone, it is important that hematology technologists include a comment reporting on the presence of these inclusions or granules. A clinical investigation with further hematologic and genetic studies may then appropriately be considered.Many of the disorders with atypical neutrophil cytoplasmic granules are also associated with platelet abnormalities, particularly giant platelets (lower photograph).Therefore, when atypical granules are recognized, scanning of the peripheral blood smear for atypical platelets may be revealing. These observations serve as readily identifiable markers for acquired and genetic human maladies, and as a guide for unraveling the reasons for a patient's suffering and impaired health.

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