Haemophilus Information and Courses from MediaLab, Inc.
These are the MediaLab courses that cover Haemophilus and links to relevant pages within the course.
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| Specimen Handling and Storage The stability of the CSF sample varies depending on the procedures ordered.
Cell counts are ALWAYS STAT and should be performed within 30 - 60 minutes for best results.
Samples should be left at room temperature for no longer than one hour and refrigerated following testing.
Refrigeration is not recommended for culture specimens since fastidious organisms such as Haemophilus influenzae and Neisseria meningitidis may not survive the cold temperature.
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| The most frequent cause of bacterial meningitis in older adults is: | View Page |
| Which of the following organisms are gram positive: | View Page |
| Which of the following media contains the X and V factors necessary for the growth of Haemophilus influenzae: | View Page |
| Which one of the following organisms is typically urease negative: | View Page |
| On sheep blood agar Haemophilus influenzae may exhibit satellite formation around all but which of the following organisms: | View Page |
| Match the organisms on the right with culture medium: | View Page |
| Which of the following growth factor(s) is necessary for the proper culture of Haemophilus influenzae: | View Page |
| Bacitracin susceptibility is useful for which two of the following: | View Page |
| Sheep blood agar contains inhibitors to which of the following organisms: | View Page |
| A Brown and Brenn gram stain was performed on one of the tissue biopsy specimens. Organisms were seen as shown in the photograph. Based on the history and the appearance of the bacteria, the most likely identification is: | View Page |
| Eikenella - catalase & oxidase Eikenella corrodens (E) belongs to the HACEK group of miscellaneous gram-negative bacilli which includes Haemophilus aphrophilus (H), Actinobacillus actinomycetemcometans (A), Cardiobacterium hominis (C) and Kingella kingae (K).Cytochrome oxidase and catalase are two rapid tests that help separate the several members of this group.Eikenella corrodens shows cytochrome oxidase activity, but not catalase activity.
The positive oxidase reaction separates E. corrodens from Haemophilus aphrophilus and Actinobacillus actinomycetemcomitans, which are both negative.A. actinomycetemcomitans is also catalase positive, an additional characteristic separating it from E. corrodens, which is negative.As Kingella kingae is also oxidase positive and catalase negative, other tests are needed for differentiation. K. kingae produces acid from glucose and maltose (E. corrodens is asaccharolytic). | View Page |
| A bacterial isolate that produces pitting of the agar and has a bleach-like odor is probably E. corrodens. Closely related species that must be ruled out include: | View Page |