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Gram negative diplococci Information and Courses from MediaLab, Inc.

These are the MediaLab courses that cover Gram negative diplococci and links to relevant pages within the course.

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Current Topics in Clinical Microbiology
A 25 year-old female presented in the emergency room with an acute urethral discharge of 2 days duration. A smear for gram stain was obtained (see accompanying image). Many polymorphonuclear leukocytes and intracellular and extracellular gram negative diplococci were observed. Based on the clinical history and the gram stain observation, a diagnosis of gonorrhea can be made.View Page

Reading Gram Stained Direct Smears
Gram negative diplococci with flattened adjacent sides within white cells may be diagnostically significant, and should be reported on the work card.View Page
Gram negative diplococci

When gram negative diplococci with flattened, adjacent sides occur within white cells, it may indicate the presence of Neisseria. An example of diplococci with flattened adjacent sides, probably representing Neisseria is shown by the arrow.

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The cell indicated by the arrow is a white blood cell containing gram negative diplococci.View Page
Recording Bacterial Characteristics

The Gram stain reaction, shape, and arrangement of bacteria, and the presence or absence of intracellular organisms must be noted on the worksheet.Examples:Gram positive cocci in chains are present.Gram negative diplococci, intracellular, are present within white blood cells.Quantitate by approximating the average number of each cell type seen in 10 oil immersion fields, and record as:Many = More than 15/fieldModerate = 4-15/fieldFew = 1-3/fieldOccasional = 2-10/10 fieldsRare = 0-2/10 fields

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