Gonorrhoeae Information and Courses from MediaLab, Inc.
These are the MediaLab courses that cover Gonorrhoeae and links to relevant pages within the course.
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| Which of the following is used as the indicator in the rapid carbohydrate utilization tests: | View Page |
| The slide coagulase test is a rapid method for identifying which of the following organisms. | View Page |
| The Thayer-Martin agar plate seen in this illustration exhibits marked growth. The most likely organism found here would be: | View Page |
| A 25 year-old female presented in the emergency room with an acute urethral discharge of 2 days duration. A smear for gram stain was obtained (see accompanying image). Many polymorphonuclear leukocytes and intracellular and extracellular gram negative diplococci were observed.
Based on the clinical history and the gram stain observation, a diagnosis of gonorrhea can be made. | View Page |
| The positive oxidase reaction as shown in the photograph (yellow arrow) rules out the following two look-alike organisms of N. gonorrhoeae: | View Page |
| The Superoxol Test Superoxol is an additional spot test that may be helpful in the presumptive identification of N. gonorrhoeae.Superoxol is 30% hydrogen peroxide, in contrast to the 3% solution that is used in the catalase test.Other Neisseria species and Moraxella catarrhalis are either negative for this test or give a weak, delayed reaction. | View Page |
| The carbohydrate utilization reaction seen in the QuadFerm system shown in the picture provides a definitive identification of N. gonorrhoeae: | View Page |
| Review 2 Smith KR, Fisher HC III, Hook, EW III: Prevalence of fluorescent monoclonal antibody-nonreactive Neisseria gonorrhoeae in five North American sexually transmitted disease clinics.J Clin Microbiol 34:1551-1552, 1996We compared a direct fluorescent monoclonal antibody (DFA) test with alternative enzymatic and fermention tests for identifying presumptive gonococcal isolates in a systematic sample from patients attending five sexually transmitted disease clinics in five cities.Fourteen (2.5%) of 556 isolates from three clinics were nonreactive with the DFA confirmatory reagent and reactive by both the Quad-Ferm and Rapid NH tests. The prevalence of DFA-nonreactive Neisseria gonorrhoeae isolates varies geographically and is independent of local methods for the identification of possible gonococci.On the basis of our findings, we recommend that for use in medicolegal and other instances in which a diagnosis of gonorrhea has the potential to have far-reaching effects, it is appropriate to test DFA reagent-nonreactive, oxidase-positive, gram-negative diplococci by alternative methods of gonococcal confirmation.Although the prevalence of such isolates could change, the fluorescent monoclonal antibody confirmation reagents remain useful for many clinical situations. Their ease of use and ready applicability for screening large numbers of isolates make them useful for many laboratories. | View Page |
| The recovery of an oxidase-positive, gram-negative diplococcus that tests DFA-nonreactive should be tested by alternative methods when establishing a fool-proof diagnosis of gonorrhea. | View Page |
| Identification of bacteria Identification of bacteria in direct smears may be of lifesaving importance. For example, a rapid diagnosis of bacterial meningitis, made after examining a gram stained smear of the patient's cerebrospinal fluid, allows the physician to begin treatment immediately.The appearance of bacteria on gram stained smears is suggestive of a certain species, but identification may not be made on the basis of the stain alone. An exception to this rule is the presence of gram negative intracellular diplococci from a male urogenital specimen, which is presumptive identification of Niesseria gonorrhoeae.In addition, culture results can be correlated with the direct smear report.
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