Four- Information and Courses from MediaLab, Inc.
These are the MediaLab courses that cover Four- and links to relevant pages within the course.
Learn more about laboratory continuing education for medical technologists to earn CE credit for AMT, ASCP, NCA, and state license renewal and recertification. Or get information about laboratory safety and compliance courses that deliver cost-effective OSHA safety training and continuing education to your laboratory's employees.
| Case Study Four- Antibody Panel Antibody panel resultsResults of the antibody panel show reactions at immediate spin and AHG with varying strengths. The pattern at IS matches P1. Remember that varied strengths can mean multiple antibodies, dosage or both.There are not enough rule-out cells to rule anything out with 3 negative reactions. You can use panel cells that reacted at IS and are negative at AHG for rule-out. Use cells 4 and 10 for rule-outs. Antibodies that have no rule-outs from this panel are: C, E, Cw, Kpa, Jsa, Fya, Lea, M,s, P1, and Lua. Cw, Kpa, Jsa, and Lua are usually not present on panels and fall under the "unable to rule out" catagory. C,E, Fya and s are clinically siginificant and should have further testing done to rule-out or rule-in these antibodies. Lea , P1 and M tend to react at IS, so if the pattern of reactivity is compared to the reaction pattern at IS, there is a match for P1.A selected cell panel was then performed. | View Page |
| Case Study Four- Selected Cell Panel Cells 5 and 8 can be used for rule-out cells. Jkb, Lea, M, N, and s still need more rule-out cells. P1, C, E and Fya have no cells for rule-out.Running an enzyme panel would help to enhance Jkb, Lea,P1 C and E if these antibodies are present.If M,N,s and Fya are present, no reaction would be seen because these antigens are destroyed by enzymes. | View Page |
| Case Study Four- Enzyme Panel C is enhanced: reactions seen on cells 1,2,and 5 E is enhanced: reactions seen on cells 3 and 6 P1 is enhanced: pattern of reactivity at IS phase matches that of P1. Jkb not reacting: cells 7,8 and 10, so not present Lea not reacting: cell 7, so not present M, N, and s will not react with enzyme treated cells. The enzyme panel was used to remove the potential reactivity of these antibodies to help further identify other antibodies present.Another selected cell panel can be run to eliminate M,N and s. | View Page |
| Case Study Four- Selected Cell Panel 2 No reactions were seen with panel cell 1 and 2. This rules out M,N,and s.Notice that C, E, Fya, and P1 are not present on these cells so as not to interfere with ruling-in or ruling-out of the remaining antibodies.ConclusionThrough the processes of ruling-in and ruling-out and matching of reaction patterns, the antibodies that are identified are C,E, Fya, and P1.If the patient has not been transfused in the past 3 months, antigen typing for Fya may be done to further confirm the presence of Fya.Elution and autoadsorptions may also be necessary to confirm the presence of C,E, Fya and P1. | View Page |