Ethylenediaminetetraacetic Information and Courses from MediaLab, Inc.
These are the MediaLab courses that cover Ethylenediaminetetraacetic and links to relevant pages within the course.
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| Specimen Collection and Handling Some global specimen collection and handling issues to consider include: Specimens that contain nucleated cells will be of interest in DNA methodologies while specimens lacking nucleated cells are more useful in RNA methodologies. rRNA is more stable than mRNA, which is labile and sensitive to contamination. DNA is relatively stable and can be obtained from nonviable sources. Serum or plasma obtained by standard routine venipuncture procedures can be used as long as proper site selection and decontamination occur. Standard anticoagulants such as Ethylenediaminetetraacetic Acid (EDTA) and Acid Citrate Dextrose (ACD) can be used; however avoid the use of heparin as an anticoagulant as it interferes with some polymerase chain reaction (PCR) methodologies. When using fluorescence, fasting serum or whole blood specimens should be used to decrease the interference by lipids. | View Page |
| When collecting blood samples, one anticoagulant to avoid, especially when performing PCR is: | View Page |