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Escherichia Information and Courses from MediaLab, Inc.

These are the MediaLab courses that cover Escherichia and links to relevant pages within the course.

Learn more about laboratory continuing education for medical technologists to earn CE credit for AMT, ASCP, NCA, and state license renewal and recertification. Or get information about laboratory safety and compliance courses that deliver cost-effective OSHA safety training and continuing education to your laboratory's employees.

Laboratories Individuals

CLIA Microbiology / Serology Review
Which of the following organisms is the most common cause of acute cystitis:View Page
The most frequent cause of bacterial meningitis in older adults is:View Page
Which of the following organisms is an obligate intracellular parasite:View Page
Which one of the following organisms is typically urease negative:View Page
The oxidase test is used to presumptively identify:View Page
Which of the following organisms is most likely to be associated with gas gangrene:View Page

Current Topics in Clinical Microbiology
The colonies shown in the blood agar (upper) and MacConkey agar (lower) biplate are a 24 hour growth from an aerobic blood culture bottle that became positive at 12 hours after inoculation. The appearance of the colonies on MacConkey agar rules out the following two bacterial species:View Page
The reactions seen in the portion of the API strip shown in the photograph, effectively rules out Escherichia coli.View Page
Clinical isolates of Escherichia coli and Klebsiella pneumoniae may possess ESBL activity. Therefore, clinical laboratories should be screening all clinically significant isolates of these two species.View Page

Introduction to Bioterrorism
Category B Agents in Food and Water

A subset of Category B agents comprise pathogens that are food or waterborne. These pathogens include but are not limited to: Salmonella species Shigella dysenteriae Escherichia coli 0157:H7 Vibrio chloerae Cryptosporidium parvum

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Packaging and Shipping Infectious Materials
Category A Definition and Examples

A category A infectious substance is in a form that is capable of causing permanent disability or life-threatening or fatal disease in otherwise healthy humans or animals when exposure to it occurs. Exposure would occur if the substance were released from its protective packaging and a human or animal came into contact with it. Some examples of category A infectious substances include: Bacillus anthracis (cultures only) Brucella abortus (cultures only) Brucella melitensis (cultures only) Burkholderia mallei (cultures only) Clostridium botulinum (cultures only) Dengue virus (cultures only) Escherichia coli, verotoxigenic (cultures only) Ebola virus Francisella tularensis (cultures only) Hantaviruses causing hemorrhagic fever with renal syndrome Herpes B virus (cultures only) Human immunodeficiency virus (cultures only) Lassa virus Mycobacterium tuberculosis (cultures only) Poliovirus (cultures only) Rabies and other lyssaviruses (culture only) Shigella dysenteriae type I (cultures only) West Nile virus (cultures only) Yersinia pestis (cultures only)This is not an exhaustive list. Sometimes, deciding on the classification of an infectious substance requires professional judgement and involves knowing the medical history or symptoms of the source patient or animal and/or knowing the local epidemiological conditions at the time the patient specimen or culture was obtained. If there is doubt as to whether or not a substance meets the criteria of category A, it must be treated as a category A substance for shipping.

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Reading Gram Stained Direct Smears
Quality Control Smears

Quality control smears are stained at the same time as the patient's slides.They must be examined before you examine the patient's slides.If properly stained, the Staphylococcus aureus on the positive control slide will stain blue, or gram positive.The Escherichia coli on the negative control slide will stain red, or gram negative.If these slides are not stained properly, the problem must be resolved and new slides made before the patient specimen is examined.

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The smears used for quality control are: (Choose ALL of the correct answers)View Page

Reading Gram Stained Smears From Cultures
Microscopic Appearance of Control Slides

The microscopic appearance of control slides must also be checked. The Gram positive control, Staphylococcus aureus, should stain blue, and the Gram negative control, Escherichia coli, should stain pink.

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