Elution Information and Courses from MediaLab, Inc.
These are the MediaLab courses that cover Elution and links to relevant pages within the course.
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| Is It a Cold or a Warm Autoantibody? Cold antibody Immediate spin screen and panel cell reactions will be postive (W+ to 4+). The auto control may also be positive. AHG reactions may be weakly positive if the cold antibody is bound strongly to the red cells. Prewarming should prevent binding from occuring. So, prewarm panels and tests should have negative reactions.Warm antibody Immediate spin screen, panel cell and auto control usually not positive. AHG reactions will be positive including auto control (W+ to 4+). Prewarming of sample and reagents will not change positive reactions since they react best at 37°C and AHG phase. So, reactions will still be positive. Elution and autoadsorption techniques may be used to help further identify the antibody or to help identify other clinically significant antibodies that may be present.AutoadsorptionAutoadsorption is a technique that involves adsorbing unbound autoantibody from the patient's serum using the patient's own red cells. Once the autoantibody is removed, then testing can be performed to determine if any clinically significant antibodies are present. | View Page |
| Case Study Four- Selected Cell Panel 2 No reactions were seen with panel cell 1 and 2. This rules out M,N,and s.Notice that C, E, Fya, and P1 are not present on these cells so as not to interfere with ruling-in or ruling-out of the remaining antibodies.ConclusionThrough the processes of ruling-in and ruling-out and matching of reaction patterns, the antibodies that are identified are C,E, Fya, and P1.If the patient has not been transfused in the past 3 months, antigen typing for Fya may be done to further confirm the presence of Fya.Elution and autoadsorptions may also be necessary to confirm the presence of C,E, Fya and P1. | View Page |
| The use of cells with known blood groups to confirm ABO typing is known as: | View Page |
| In preparing red cells for any elution method , one must be particularly careful to: | View Page |
| The Kleihauer-Betke test is used to: | View Page |