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Electrophoretic Information and Courses from MediaLab, Inc.

These are the MediaLab courses that cover Electrophoretic and links to relevant pages within the course.

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Laboratories Individuals

CLIA Chemistry / Urinalysis Review
Increases in the MB fraction of CK is associated with:View Page

CLIA General Laboratory Review
Electrophoretic separation fundamentally relies on:View Page

Electrophoresis
The pI of a protein is 9.2. This protein is placed in an electrical field where a buffer sets the pH at 10.0. Select the correct statement regarding the electrophoretic migration of this protein.View Page
Types of Support Media

For electrophoretic separation of solutes, the sample of solutes is placed on a gel or membrane in contact with buffer for separation. Common gels are cellulose acetate, agarose, and polyacrylamide gels. These gels are formed into sheets, slabs, or inserted into columns or tubes. The gel can be positioned horizontally or vertically.Cellulose is chemically reacted with acetic anyhdride to form a cellulose acetate gel. Because cellulose requires soaking before sample application and a clearing step for detection of separated solutes or bands, agarose gel is more often used than cellulose acetate gel for clinical electrophoresis.

View Page
Wick flow is caused by movement of the buffer ions into the medium when there has been a loss of moisture in the medium due to heat generation.View Page


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