Electrodes Information and Courses from MediaLab, Inc.
These are the MediaLab courses that cover Electrodes and links to relevant pages within the course.
Learn more about laboratory continuing education for medical technologists to earn CE credit for AMT, ASCP, NCA, and state license renewal and recertification. Or get information about laboratory safety and compliance courses that deliver cost-effective OSHA safety training and continuing education to your laboratory's employees.
|Ionized calcium is most commonly measured using which of the following method:||View Page|
|Match principle with instrument:||View Page|
In addition to the specimen sample, support medium and buffer for electrophoresis, a power supply, positive and negative electrodes, chamber, and identification or detection method are needed.The power supply is a source of constant voltage or current that provides energy to the electrodes. This drives the movement of the ions in the medium and results in the movement and separation of the molecules or solutes in the specimen. Control of current or voltage comes with the power source in order to make adjustments.The chamber is divided into two sections or has two reservoirs for the buffer and one electrode is placed in each. The support medium is laid over the chamber in such a way that it connects the two reservoirs. A lid or cover is placed over the chamber during electrophoresis.
|Pulsed Field Electrophoresis|
Larger fragments of DNA, > 50 kilobases (kb), cannot be separated with AGE or PAGE in routine electrophoresis systems; the gel pore sizes are too small for their migration. Fragment separation can be achieved with alternately applying the power to different pairs of electrodes. The most common method alternates the positive and negative electrodes in cycles during electrophoresis. The DNA fragments must reorient to a new field direction in each cycle. These changing pulses and reorientations separate the large size DNA fragments.Sample runs require longer time periods, some 24 hours or more, special gel boxes, different electrodes and controls for switching the electric fields during electrophoresis.
|The impedance principle shown in this illustration is best described by the following statement:||View Page|